Influenza B IgM ELISA Kit


Human influenza B IgM ELISA kit is designed for determining in-vitro quantitative amounts of human IgM antibodies against influenza B (influenza-B IgM) in serum or plasma samples. This assay has a minimum analytical sensitivity limit of 1.19 U/ml.


Influenza B viruses can be divided into two co-circulating lineages (B/Yamagata/16/88-like or B/Victoria/2/87-like viruses), this is based on their antigenic properties on the surface of the glycoprotein hemagglutinin. At present influenza B has only been found to cause human or seal infections. The influenza B virus capsid is filamentous and spherical, consisting of more than 500 surface projections of neuraminidase and hemagglutinin. The genome of the virus is around 14548 nucleotides in length and consists of eight linear negative-sense, single stranded RNA.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Influenza B antigen coated microtiter strips.
  • Enzyme Conjugate.
  • Sample Diluent.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.
  • Calibrator D (Positive Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator A (Negative Control).
  • Calibrator C (Weak Positive Control).


The minimum detection sensitivity level of IgM to influenza B (influenz B IgM) using current human influenza-B IgM ELISA kit was 1.19 U/ml. The dynamic range for this assay is 1.0 – 250.0 U/ml.


– Influenza B ELISA: IgM
– Intra-Assay-Precision: 8.2 %
– Inter-Assay-Precision: 9.6 %
– Inter-Lot-Precision: 3.3 – 9.7 %
– Analytical Sensitivity: 1.19 U/mL
– Recovery: 71 – 92 %
– Linearity: 82 – 123 %
– Cross-Reactivity: No cross-reactivity to Parainfluenza 1/2/3, RSV and Adenovirus.
– Interferences: No interferences to hemoglobin up to 8.0 mg/mL, bilirubin up to 0.3 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %


  1. Role of IgM antibodies versus B cells in influenza virus-specific immunity. Eur J Immunol. (2002) 32 (8): 2229-36. Kopf M., et al.
  2. Use of a simple, new test for virus-specific IgM to investigate an outbreak of influenza B in a hospitalised aged community. J Virol Methods. (1982) 4 (1): 9-18. Goldwater P.N., et al.
  3. Influenza virus serology–a comparative study. J Virol Methods. (1999) 78 (1-2): 163-9. Rothbarth P.H., et al.
  4. Influenza A and B virus IgG and IgM serology by enzyme immunoassays. Epidemiol Infect. (1987) 99 (1): 55-64. Koskinen P., et al.


  • Full Name: Influenza B IgM ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.19 U/ml



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