Influenza A IgG ELISA Kit

Full Name: Influenza A IgG ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.1 U/ml


There are influenza viruses A, B, C, and D which are mostly found in wild aquatic birds. Only types A and B can cause the vast majority of human diseases. These  viruses are identified by either their neuraminidase (NA) or hemagglutinin (HA).

Currently, there are 11 different NA subtypes and 18 HA subtypes recorded. Scientists have identified two distinct subtypes among humans; H3N2 and H1N1.


Human influenza A IgG ELISA kit is designed for detecting in-vitro amounts of human IgG antibodies to influenza A (influenza-A IgG) in serum and plasma samples. This assay has a minimum analytical sensitivity limit of 1.1 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Calibrator A (Negative Control).
  • Calibrator D (Positive Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Sample Diluent.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.
  • Influenza A antigen coated microtiter strips.


The minimum detection sensitivity level human IgG to to influenza A (influenza-A IgG) using current influenza A IgG ELISA kit was 1.1 U/ml. The dynamic assay range for this kit is 1.0 – 150.0 U/ml.


– Intra-Assay-Precision: 8.5 %
– Inter-Assay-Precision: 6.5 %
– Inter-Lot-Precision: 3.8 – 6.1 %
– Analytical Sensitivity: 1.09 U/mL
– Recovery: 100 – 114 %
– Linearity: 79 – 114 %
– Cross-Reactivity: No cross-reactivity to RSV, Adenovirus and Parainfluenza 1/2/3.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 88 %
– Clinical Sensitivity: 100 %


  1. A pilot study showing differences in glycosylation patterns of IgG subclasses induced by pneumococcal, meningococcal, and two types of influenza vaccines. Immun Inflamm Dis. (2014) 2 (2): 76-91. Vestrheim A.C., et al.
  2. Detection of influenza A/H1N1-specific human IgG-secreting B cells in older adults by ELISPOT assay. Viral Immunol. (2014) 27 (2): 32-8. Painter S.D., et al.
  3. Influenza A and B virus IgG and IgM serology by enzyme immunoassays. Epidemiol Infect. (1987) 99 (1): 55-64. Koskinen P., et al.
  4. Serum IgG subclass responses of humansto inactivated and live influenza-A vaccines compared to natural infections with influenza A. J Med Virol. (1990) 30 (2): 92-6. Hocart M.J., et al.


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