Influenza B IgG ELISA Kit

Full Name: Influenza B IgG ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.07 U/ml


Influenza B viruses have only been identified to infect humans and seals. At present there are two co-circulating lineages which are classified according to their antigenic properties on glycoprotein hemagglutinin surface. The two lineages are referred to either B/Victoria/2/87-like or B/Yamagata/16/88-like viruses.

The genome of the virus is approx. 14548 nucleotides long and made up of eight fragments of linear single stranded negative-sense RNA. The influenza B virus capsid is spherical and filamentous, having over 500 surface projection of hemagglutinin and neuraminidase.


Human influenza B IgG ELISA kit is a method for analysing in-vitro concentrations of human IgG antibodies to influenza B (influenza-B IgG) using plasma or serum. This assay has a minimum analytical sensitivity limit of 1.07 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Influenza B antigen coated microtiter strips.
  • Sample Diluent.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator A (Negative Control).
  • Calibrator D (Positive Control).


The minimum detection sensitivity level of human IgG against influenza B (influenza B IgG) using current influenza B IgG ELISA kit was 1.07 U/ml. The dynamic assay range for this kit is 1.0 – 175.0 U/ml.


– Intra-Assay-Precision: 9.2 %
– Inter-Assay-Precision: 8.9 %
– Inter-Lot-Precision: 4.8 – 11.0 %
– Analytical Sensitivity: 1.07 U/mL
– Recovery: 89 – 107 %
– Linearity: 71 – 123 %
– Cross-Reactivity: No cross-reactivity to Adenovirus, RSV and Parainfluenza 1/2/3.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, triglycerides up to 5.0 mg/mL and hemoglobin up to 8.0 mg/mL.
– Clinical Specificity: 89 %
– Clinical Sensitivity: 100 %


  1. Influenza A and B virus IgG and IgM serology by enzyme immunoassays. Epidemiol Infect. (1987) 99 (1): 55-64. Koskinen P., et al.
  2. Intranasal immunization with influenza antigens conjugated with cholera toxin subunit B stimulates broad spectrum immunity against influenza viruses. Hum Vaccin Immunother. (2014) 10 (5): 1211-20. Li J., et al.
  3. Laboratory and epidemiologic assessment of a recent influenza B outbreak. J Med Virol. (1988) 25 (3): 361-8. King J.C. Jr, et al.
  4. Monoclonal antibodies for the rapid diagnosis of influenza-B virus infections by ELISA: production and characterization. Clin Diagn Virol. (1995) 4 (1): 27-42. Glikmann G., et al.


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