Influenza A IgM ELISA Kit

Full Name: Influenza A IgM ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.17 U/ml


Influenza A type viruses are mainly found in wild aquatic birds, there are influenza viruses types A, B, C and D. They belong to a family which is called Orthomyxoviridae and are also more commonly referred to as the flu. It is only type A and type B which can cause majority of human diseases.

Influenza A viruses are identified by either their neuraminidase (NA) or hemagglutinin (HA). Currently, there are 11 different NA subtypes and 18 HA subtypes recorded. Scientists have identified two distinct subtypes among humans; H3N2 and H1N1.


Human influenza A IgM ELISA kit can be used for measuring in-vitro levels of human IgM antibodies against influenza A (influenza-A IgM) using plasma or serum. This assay has a minimum analytical sensitivity limit of 1.17 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Influenza A antigen coated microtiter strips.
  • Calibrator B (Cut-Off Standard).
  • Calibrator A (Negative Control).
  • Calibrator D (Positive Control).
  • Calibrator C (Weak Positive Control).
  • Enzyme Conjugate.
  • Sample Diluent.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.


The minimum detection sensitivity level of human IgM antibodies to influenza A (influenza A IgM) using current influenza A IgM ELISA kit was 1.17 U/ml. The dynamic assay range for this kit is 1.0 – 150.0 U/ml.


– Intra-Assay-Precision: 8.3 %
– Inter-Assay-Precision: 10.8 %
– Inter-Lot-Precision: 1.3 – 9.9 %
– Analytical Sensitivity: 1.17 U/mL
– Recovery: 83 – 90 %
– Linearity: 73 – 122 %
– Cross-Reactivity: No cross-reactivity to RSV, Parainfluenza 1/2/3 and Adenovirus.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %


  1. Serum IgM antibody and influenza-A infection. J Clin Pathol. (1977) 30 (8): 723-7. Buchner Y.I., et al.
  2. Influenza virus-specific neutralizing IgM antibodies persist for a lifetime. Clin Vaccine Immunol. (2014) 21 (11): 1481-9. Skountzou I., et al.
  3. Measurement of IgM responses to a subunit influenza A vaccine by sucrose-gradient centrifugation and membrane-filtration enzyme immunoassays. Biologicals. (1991) 19 (1): 17-21. Tannock G.A., et al.
  4. Influenza A and B virus IgG and IgM serology by enzyme immunoassays. Epidemiol Infect. (1987) 99 (1): 55-64. Koskinen P., et al.
  5. Morphological studies of the neutralization of influenza-virus by IgM. J Gen Virol. (1990) 71 (Pt 10): 2313-9. Armstrong S.J., et al.


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