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Influenza A IgM ELISA Kit

INTENDED USE

Human influenza A IgM ELISA kit can be used for measuring in-vitro quantitative levels of human IgM antibodies against influenza A (influenza-A IgM) using plasma or serum. This assay has a minimum analytical sensitivity limit of 1.17 U/ml.

BACKGROUND

Influenza A type viruses are mainly found in wild aquatic birds, there are influenza viruses types A, B, C and D. They belong to a family which is called Orthomyxoviridae and are also more commonly referred to as the flu. It is only type A and type B which can cause majority of human diseases. Influenza A viruses can be classified as either being neuraminidase (NA) or hemagglutinin (HA), at present there are 11 NA subtypes and 18 HA subtypes that have been discovered. However, in humans only two types of influenza A virus subtypes have been identified H3N2 and H1N1.

INFLUENZA A IGM ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Influenza A antigen coated microtiter strips.
  • Calibrator B (Cut-Off Standard).
  • Calibrator A (Negative Control).
  • Calibrator D (Positive Control).
  • Calibrator C (Weak Positive Control).
  • Enzyme Conjugate.
  • Sample Diluent.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of IgM antibodies to influenza A (influenza A IgM) using current human influenza-A IgM ELISA kit was 1.17 U/ml. The dynamic assay range for this kit is 1.0 – 150.0 U/ml.

ASSAY CHARACTERISTICS

– Influenza A ELISA: IgM
– Intra-Assay-Precision: 8.3 %
– Inter-Assay-Precision: 10.8 %
– Inter-Lot-Precision: 1.3 – 9.9 %
– Analytical Sensitivity: 1.17 U/mL
– Recovery: 83 – 90 %
– Linearity: 73 – 122 %
– Cross-Reactivity: No cross-reactivity to RSV, Parainfluenza 1/2/3 and Adenovirus.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 100 %

REFERENCES

  1. Serum IgM antibody and influenza A infection. J Clin Pathol. (1977) 30 (8): 723-7. Buchner Y.I., et al.
  2. Influenzavirus-specific neutralizing IgM antibodies persist for a lifetime. Clin Vaccine Immunol. (2014) 21 (11): 1481-9. Skountzou I., et al.
  3. Measurement of IgM responses to a subunit influenza A vaccine by sucrose-gradient centrifugation and membrane-filtration enzyme immunoassays. Biologicals. (1991) 19 (1): 17-21. Tannock G.A., et al.
  4. Influenza A and B virus IgG and IgM serology by enzyme immunoassays. Epidemiol Infect. (1987) 99 (1): 55-64. Koskinen P., et al.
  5. Morphological studies of the neutralization of influenzavirus by IgM. J Gen Virol. (1990) 71 (Pt 10): 2313-9. Armstrong S.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Influenza A IgM ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.17 U/ml

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