HbA1c ELISA Kit (Glycated Hemoglobin A1c)

Full Name: HbA1c ELISA Kit (Glycated Hemoglobin A1c)
Reactivity: Human
Sample Type: Tissue Homogenates, Plasma, Serum, Biological Fluids
Sensitivity: 37.5 pg/ml

INTRODUCTION

Glycated hemoglobin A1c (HbA1c) is present in people who have had diabetes for some time. Normally, as glucose leaves the cells and travels through the blood, it binds to haemoglobin before being absorbed by cells all throughout your body. The most commonly used tests to measure glycation are glycated haemoglobin and glycated albumin. This test will show haemoglobin that has bound to glucose, which can be measured in millimol per liter units. It is created by the nonenzymatic chemical reaction between glucose and the amino group on the N-terminal valine of the haemoglobin beta chain. It represents about 5% of the total HbA fraction present. This glycosylated form of haemoglobin is produced by the body following prolonged periods of hyperglycemia. It forms in response to high blood glucose levels, specifically when the pancreas cannot keep up with producing insulin.

To confirm whether a patient’s hemoglobin a1c is within normal range, they need to also obtain their fasting plasma glucose and postprandial plasma glucose levels on separate days. If these values are normal, then the HbA1c can be considered normal as well. However, if values of HbA1c are elevated it may indicate more serious problems such as dehydration, diabetes, or thyroid disease that should be investigated further. The normal reference range is 4.5-6%.

INTENDED USE

Human HbA1c ELISA kit can be used to measure amounts of glycated hemoglobin A1c (HbA1c) present is human serum, plasma, biological fluids and tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-HbA1c antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this HbA1c ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml.
Reactivity: Human
Sensitivity: 37.5 pg/ml
Range: 62.5 – 4000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for HbA1c, no cross reactivity or interference between HbA1c and analogues was detected.
– Recovery: Serum (86 – 98%), EDTA Plasma (87 – 103%), Heparin Plasma (86 – 104%).
– Linearity: Serum (86 – 98%), EDTA Plasma (86 – 101%), Heparin Plasma (86 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. HbA1c: a review of analytical and clinical aspects. Ann Lab Med. (2013) 33 (6): 393-400. Weykamp C.
2. Evaluation of glycated albumin (GA) and GA/HbA1c ratio for diagnosis of diabetes and glycemic control: A comprehensive review. Crit Rev Clin Lab Sci. (2017) 54 (4): 219-232. Yazdanpanah S., et al.
3. The long and winding road to optimal HbA1c measurement. Clin Chim Acta. (2013) 15; 418:63-71. Little R.R. and Rohlfing C.L.
4. Diabetes, Glycated Hemoglobin, and Risk of Cancer in the UK Biobank Study. Cancer Epidemiol Biomarkers Prev. (2020) 29 (6): 1107-1119. Peila R. and Rohan T.E.

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