IAPP ELISA Kit (Islet Amyloid Polypeptide)

Full Name: IAPP ELISA Kit (Islet Amyloid Polypeptide)
Reactivity: Human
Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma
Sensitivity: 9.375 pg/ml

INTRODUCTION

Insulin, insulin fragments, or proinsulin were assumed to be the main protein components of islet amyloid polypeptide (IAPP) deposits at first, but two groups independently discovered that the main protein component is a 37-residue polypeptide neuropancreatic hormone. The human form is a naturally occurring, intrinsically disordered protein whose abnormal aggregation into amyloid fibrils is a pathological feature in type 2 diabetes and Alzheimer’s disease. Long, disorganised human pancreatic polypeptides can be broken down by proteolytic cleavage to form alpha-helical filaments. In humans and other mammals, the pancreas secretes the protein known as amyloid polypeptide. Amyloid polypeptides are highly similar to insulin and fragments thereof are not detected as the major protein species in CSF of neurodegenerative disease patients. Fragments of amyloid polypeptides are detectable by ELISA methods in plasma as a novel protein species without detection as a major protein species in CSF.

Islet amyloid polypeptide is one of the major secretory products of β-cells of the pancreatic islets of Langerhans. It is a potential regulatory peptide that inhibits insulin and glucagon secretion locally in the islets as well as at distant sites. In humans, there is one unit for every six units of insulin produced by the pancreas. This means that when a person has type 1 or type 2 diabetes, IAPP levels will be lower than normal in their blood. IAPP has putative functions both locally in the islets and at distant targets like reducing appetite and food intake. The function in the pancreas includes inhibiting insulin secretion from β -cells, “acting as an antioxidant, and a promoter of cell growth”.

INTENDED USE

Human IAPP ELISA kit can detect amounts of islet amyloid polypeptide (IAPP, Amylin, Diabetes-associated peptide, DAP, insulinoma amyloid) present is human plasma, serum, biological fluids and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti-IAPP antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this IAPP ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IAPP, no cross reactivity or interference between IAPP and analogues was detected.
– Recovery: Serum (91 – 103%), EDTA Plasma (88 – 100%), Heparin Plasma (87 – 95%).
– Linearity: Serum (87 – 104%), EDTA Plasma (87 – 101%), Heparin Plasma (85 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

Islet Amyloid Polypeptide: Structure, Function, and Pathophysiology. J Diabetes Res. (2016) 2016: 2798269. Akter R., et al.
Metabolic stress, IAPP and islet amyloid. Diabetes Obes Metab. 2012 Oct;14 Suppl 3:68-77. Montane J., et al.
IAPP and type 1 diabetes: implications for immunity, metabolism and islet transplants. J Mol Endocrinol. (2018) 60 (2): R57-R75. Denroche H.C. and Verchere C.B.

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