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Hamster TNF-Alpha ELISA Kit (Tumor Necrosis Factor Alpha)

Full Name: Hamster TNF-Alpha ELISA Kit (Tumor Necrosis Factor Alpha)
Reactivity: Hamster
Sample Type: Plasma, Biological Fluids, Serum, Tissue Homogenates
Sensitivity: 1.875 ng/ml

INTRODUCTION

TNF-alpha is a 157-amino-acid protein with a molecular mass of 17 kDa. It is produced by natural killer cells, T lymphocytes, activated monocytes, and macrophages, and is primarily present in the form of a homotrimer in different solutions. The human TNF alpha gene was cloned in 1985. It is approximately 3 kilobases long, contains 4 exons, and is located on chromosome 6p21.3. Lymphotoxin alpha (LTA), which is related to the last exon. The three’-UTR of the primary untranslated region (3′-UTR) has an AU-rich segment.

TNF-alpha is essential for regulating the number of precursor osteoclasts in the bone marrow since doing so can enhance the RANK signalling pathway. It also inhibits the recruitment of tyrosine kinase receptors and reduces phosphorylation of CREB and ERK2 in order to attenuate RANK ligand signalling when it binds to its receptor found on osteoclast precursors. It acts on TCR in order to induce signalling cascades that lead to inflammation, autoimmune response and cell death.

INTENDED USE

Hamster TNF-alpha ELISA kit can be used for analysing concentrations of tumor necrosis factor alpha (TNF-α, DIF, TNF-alpha, TNFA, TNFSF2) in hamster plasma, serum, biological fluids and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with TNF-alpha antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For the following hamster TNF-alpha ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 3.125, 6.25, 12.5, 25, 50, 100, 200 pg/ml.
Reactivity: Hamster
Sensitivity: 1.875 pg/ml
Range: 3.125 – 2000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TNF-alpha, no cross reactivity or interference between TNF-alpha and analogues was detected.
– Recovery: Serum (87 – 101%), EDTA Plasma (89 – 98%), Heparin Plasma (85 – 95%).
– Linearity: Serum (89 – 105%), EDTA Plasma (87 – 103%), Heparin Plasma (88 – 103%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. High-dose tumor necrosis factor alpha produces an impairment of hamster diaphragm contractility. Attenuation with a prostaglandin inhibitor. Am J Respir Crit Care Med. (1996) 153 (5): 1611-5. Wilcox P., et al.
  2. Anticancer activity of Vicenin-2 against 7,12 dimethylbenz[a]anthracene-induced buccal pouch carcinoma in hamsters. Biochem Mol Toxicol. (2021) 35 (3): e22673. Li Y., et al.
  3. TNF-alpha-mediated signal transduction pathway is a major determinant of apoptosis in dilated cardiomyopathy. J Cell Mol Med. (2010) 14 (7): 1988-97. Das S., et al.
  4. Effect of topical chamomile on immunohistochemical levels of IL-1β and TNF-α in 5-fluorouracil-induced oral mucositis in hamsters. Cancer Chemother Pharmacol. (2013) 71 (2): 293-9. Curra M., et al.

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