Growth Hormone 2 ELISA Kit (GH2)

Full Name: Growth Hormone 2 ELISA Kit (GH2)
Reactivity: Human
Sample Type: Biological Fluids, Plasma, Tissue Homogenates, Serum
Sensitivity: 46.875 pg/ml

INTRODUCTION

Growth hormone 2 (GH2) is released by the placenta during pregnancy, it is important in fetal growth. This hormone belongs to the family of somatotropin/prolactin, it also the predominant form growth control hormone. This is synthesised mainly within the anterior pituitary and it is stored in the form of secretory granules. It is present during pregnancy and is released from the placenta. This is synthesised mainly within the anterior pituitary and it is stored in the form of secretory granules.

The GH2 gene is located on chromosome 17 of the growth hormone locus. There are 5 genes that display a high level of sequence identity. GH1 is the most dominant isoform, especially amongst the ones that display similar somatogenic and reduced lactogenic activities. Mutations of this gene can result in deficiencies in placental growth hormone/lactogen and somatogenic/lactogenic activities.

The product of the Growth hormone 2 gene is a glycoprotein consisting of 191 amino acids that is a homodimeric molecule with five disulfide bonds. The linker region, which consists of 52 amino acids, connects the N- and C-terminal ends of the protein, which has an average molecular weight of 16,849 Da. The GH2 gene has four exons, with the first three encoding for an intracellular domain containing signal peptidase I (SPI), intermediate filament binding protein 2 (IFBP2), GTPase activating protein (GAP) and a domain encoded by exon 4. The fourth exon encodes for a secreted protein of unknown function.

INTENDED USE

Human growth hormone 2 ELISA kit is designed for determining concentrations of growth hormone 2 (GH2, GHL, GHV, placenta-specific growth hormone) using human samples of plasma, biological fluids, serum and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-GH2 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this growth hormone 2 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 78.125, 156.25, 312.5, 625, 1250, 5000 pg/ml.
Reactivity: Human
Sensitivity: 46.875 pg/ml
Range: 78.125 – 5000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for GH2, no cross reactivity or interference between GH2 and analogues was detected.
– Recovery: Serum (86 – 100%), EDTA Plasma (85 – 102%), Heparin Plasma (86 – 101%).
– Linearity: Serum (85 – 99%), EDTA Plasma (87 – 96%), Heparin Plasma (80 – 95%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Incorporation of human growth hormone-2 into proteoliposome enhances tissue regeneration with anti-oxidant and anti-senescence activities. Rejuvenation Res. (2015) 18 (1): 20-9. Kim S.H., et al.
2. Complex signatures of locus-specific selective pressures and gene conversion on Human Growth Hormone/Chorionic Somatomammotropin genes. Hum Mutat. (2008) 29 (10): 1181-93. Sedman L, et al.
3. Developmental programming of growth: genetic variant in GH2 gene encoding placental growth hormone contributes to adult height determination.Placenta. (2013) 34 (11): 995-1001. Timasheva Y, et al.
4. A review of maternal and fetal growth factors in diabetic pregnancy. Curr Diabetes Rev. (2010) 6 (2): 116-25. Higgins M. and Mc Auliffe F.

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