Presepsin ELISA Kit (Presepsin/Soluble CD14)

Full Name: Presepsin ELISA Kit (Presepsin/Soluble CD14)
Reactivity: Human
Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids,
Sensitivity:  0.094 ng/ml

INTRODUCTION

Presepsin (soluble CD14) is a 13kDa protein produced by cleavage of the CD14 receptor. It is generated when the membrane-bound CD14 receptor is proteolytically cleaved in response to bacterial lipopolysaccharides and other microbial compounds. It has also been found as a valuable biomarker, the levels of which may be utilised to identify sepsis. Proteolytic cleavage of the membrane-bound CD14 receptor releases a soluble fraction, which is proteolytically cleaved further.

Presepsin levels in the blood rise nearly twofold in the presence of sepsis, peaking within 24 hours after diagnosis. After 24 hours, the levels immediately reach a stable state and then progressively fall. Serum levels have been demonstrated to stay steady for up to 5 days following an initial septic insult, with no difference between known survivors and those who died during this period. The major clearance pathway for presepsin is via hepatic metabolism.

Presepsin has a molecular weight of 11,370 Daltons and consists of an amino acid chain folded into four domains (A to D). The A domain runs along the N-terminus; it contains three disulfide bridges and a single thiol group in its cysteine residue at position 5. The B domain starts at Val-330; it also contains six disulfide bonds with two thiol groups in As p-296 and Asp-329, a single thiol group in Glu-327, and a serine residue at position 16. The C domain starts at Ala-337; it contains two disulfide bonds with one thiol group in Arg-346 and an aspartic acid residue at position 41. The D domain begins three residues before the N terminus; it also contains four disulfide bonds and an aspartic acid residue at position 53.

INTENDED USE

Human presepsin ELISA kit is intending for measuring levels of presepsin (soluble CD14 subtype) present in human samples of plasma, serum, biological fluids and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-presepsin antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this presepsin ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for presepsin, no cross reactivity or interference between presepsin and analogues was detected.
– Recovery: Serum (93 – 99%), EDTA Plasma (92 – 99%), Heparin Plasma (86 – 99%).
– Linearity: Serum (85 – 105%), EDTA Plasma (82 – 98%), Heparin Plasma (84 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Presepsin:albumin ratio and C-reactive protein:albumin ratio as novel sepsis-based prognostic scores: A retrospective study. Wien Klin Wochenschr. (2020) 132 (7-8): 182-187. Kaplan M., et al.
2. Presepsin: A promising biomarker for the detection of bacterial infections. Biomed Pharmacother. (2019) 111: 649-656. Memar M.Y. and Baghi H.B.
3. The combination of procalcitonin and C-reactive protein or presepsin alone improves the accuracy of diagnosis of neonatal sepsis: a meta-analysis and systematic review. Crit Care. (2018) 22 (1): 316. Ruan L., et al.

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