GIP ELISA Kit (Gastric Inhibitory Polypeptide)

INTENDED USE

Human GIP ELISA kit can detect amounts of gastric inhibitory polypeptide (GIP, Incretin, Glucose-dependent insulinotropic) present is human plasma, serum, biological fluids and tissue homogenates.

INTRODUCTION

Found throughout the gut lining, these K-cells kick off a response that helps manage how your body handles incoming glucose. Though once framed mainly as a suppressor of stomach acidity, recent understanding shifts its function toward energizing insulin release based on dietary intake. Present in the duodenum and early sections of the jejunum, these cells form key stations for chemical messaging after meals. By 2026, researchers view its core job not through acid reduction but via prompt insulin generation tied to sugar levels. This “incretin effect” helps the body handle rising blood sugar after meals, keeping overall metabolism balanced.

Far from just acting in the pancreas, GIP also influences how fats are handled in the body. Unlike GLP-1, which helps people feel full to reduce overall weight, GIP works differently – its receptors are active in fat cells, not only in the pancreas. When insulin is present, GIP supports an enzyme called lipoprotein lipase, which makes it easier for fatty acids to enter cells. Because of this process, those fatty acids tend to be stored more effectively as triglycerides. Still, new discoveries in medicine suggest GIP alongside GLP-1 may boost calorie burn and lower body-wide swelling when taken in standard amounts. Because of this, scientists now design treatments using two active agents – one that makes up for GIP’s role in lessening unwanted reactions from other blood-sugar drugs while strengthening how well glucose levels stay under control.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-GIP antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Stop Solution
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this GIP ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 46.875, 93.75, 187.5, 375, 755, 1500, 3000 pg/ml.
Reactivity: Human
Sensitivity: 28.125 pg/ml
Range: 46.875 – 3000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for GIP, no cross reactivity or interference between GIP and analogues was detected.
– Recovery: Serum (88 – 96%), EDTA Plasma (87 – 104%), Heparin Plasma (91 – 105%).
– Linearity: Serum (86 – 102%), EDTA Plasma (82 – 100%), Heparin Plasma (80 – 93%).
– Precison Intra-Assay: CV < 5%.
– Precison Inter-Assay: CV < 5%.

REFERENCES

1. GIP and GLP-1, the two incretin hormones: Similarities and differences. Fukushima M, Yabe D.J Diabetes Investig. (2010) 1 (1-2):8-23. Seino Y., et al.
2. GIP’s involvement in the pathophysiology of type 2 diabetes. Peptides. (2020) 125:170178. Christensen M.B., et al. Review.
3. Glucose-dependent insulinotropic polypeptide (GIP) and cardiovascular disease. Peptides. (2020) 125 :170174. Heimbürger S.M., et al.
4. Recent advances of GIP and future horizons. Peptides. (2020) 125: 170230. Holst J.J. and Rosenkilde M.M.

ADDITIONAL INFORMATION

• Full Name: GIP ELISA Kit (Gastric Inhibitory Polypeptide)
• Reactivity: Human
• Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids
• Sensitivity: 28.125 pg/ml

OTHER RELATED ELISA KITS

• Insulin ELISA Kit
• GLP-1 ELISA Kit
• Cortisol ELISA Kit