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GLP-1 Active ELISA Kit (Glucagon-Like Peptide-1 Active)

INTENDED USE

Human GLP-1 active ELISA kit is a reliable quantitative procedure for measuring active glucagon-like peptide-1 (GLP-1 active), this includes GLP-1 (7-36) amide and GLP-1 (7-37) specifically in human plasma (EDTA). This assay has a minimum sensitivity limit of 0.1 pmol/l.

BACKGROUND

Glucagon-like peptide-1 (GLP-1), main function is to regulate glucose homeostasis. It is also secreted in response to food intake. It belongs to the incretins family of gastrointestinal hormones, these have the ability to increase the levels of insulin that is released from the beta cells of the islets of Langerhans after food intake. It is also able to inhibit  glucagon  release  from  the  alpha  cells  of  the  islets  of Langerhans.

One of the major source for active GLP-1 in the periphery is the intestinal L cell. These have the ability to secrete it as a gut hormone, on the other hand the main source in the brain is the nucleus of the solitary tract and this is a widely distributed set of projection neurons. The biologically active forms include GLP-1 (7-37) and GLP-1 (7-36) NH2. These can be formed as a result of selective cleavage of the pro-glucagon molecule.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Plate: Wells coated with anti GLP-1 rabbit IgG.
  • Labelled Antibody (30x Concentrated).
  • Standards: Concentrations 0, 1.25, 2.5, 5, 10, 20, 40, 80.0 pmol/l.
  • EIA Buffer.
  • Antibody Labelling Solution.
  • TMB Chromogen Solution.
  • Stop solution: H2SO4 (1N).
  • Wash Buffer (40x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of human active glucagon-like peptide-1 (GLP-1 active) using current GLP-1 active ELISA kit was approximately 0.1 pmol/l. The dynamic range for this assay is 1.25 – 80.0 pmol/l.

ASSAY CHARACTERISTICS

– Glucagon-Like Peptide-1 (GLP-1 active, GLP-1 [7-36], GLP-1 [7-37]): ELISA
– Recovery: 82.0 – 99.6%
– Intra-Assay: 5.4 – 5.5%
– Inter-Assay: 7.1 – 10.9%
– Specificity: GLP-1 (7-36)amide (100 %), GLP-1 (7-37)(100 %), GLP-1 (1-37)(0.32 %),
GLP-1 (9-36) amide, GLP-2, Glucagon, Human GIP, Mouse GIP (< 0.1 %).
– Sensitivity: 0.10 pmol/L.

REFERENCES

  1. Incretin hormones–an update. Scand J Clin Lab Invest Suppl. (2001) 234:75-85. Review. Holst J.J. and Orskov C.
  2. Mechanisms for the cardiovascular effects of glucagon-like peptide-1. Acta Physiol (Oxf). (2016) 216 (3): 277-313. Review. Poudyal H.
  3. Active metabolite of GLP-1 mediates myocardial glucose uptake and improves left ventricular performance in conscious dogs with dilated cardiomyopathy. Am J Physiol Heart Circ Physiol. (2005) 289 (6): H2401-8. Nikolaidis L.A., et al.

ADDITIONAL INFORMATION

  • Full Name: GLP-1 Active ELISA Kit (Glucagon-Like Peptide-1 Active)
  • Reactivity: Human
  • Sample Type: Plasma (EDTA)
  • Sensitivity: 0.1 pmol/l

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