Cortisol ELISA Kit
INTENDED USE
Human cortisol ELISA kit is a reliable quantitative procedure for measuring cortisol in human serum and plasma. This assay has a minimum sensitivity detection of 3.79 ng/ml.
BACKGROUND
Cortisol (hydrocortisone, compound F) is a corticosteroid or glucocorticoid. It is produced in the zona fasciculate and the zona reticularis of the adrenal cortex. Over 90% is bound to cortisol-binding globulins (CBG) and around 7% is bound to albumin, only 1-3% is unbound (this represent the active form of cortisol).
Among the products that are released by the adrenal cortex, only cortisol has been known to regulate ACTH secretion, for example the release of ACTH is inhibited through a negative feedback loop as the level of free-cortisol (non-protein bound) in blood rises and the reverse happens when the levels are subnormal. The main biological effects include; deposition of liver glycogen, effect on fat metabolism, promotion of gluconeogenesis, anti-inflammatory action and the increase in blood glucose concentration when carbohydrate utilization has been reduced.
Measurement of cortisol is a useful indicator in diseases with abnormal glucocorticoid production e.g. Cushing Syndrome (hypercortisolism), Addison’s disease or even secondary adrenal insufficiency (hypocortisolism). Due to the diurnal fluctuation levels it may be necessary to take several samples that are going to be used for an individual profile or used during dynamic tests such as ACTH stimulation and dexamethasone suppression tests.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Wells coated with anti-cortisol antibody.
- Calibrators 0-5: Concentrations 0, 10, 30, 90, 270, 800 ng/ml.
- Control 1 (Low) And Control 2 (High).
- Enzyme Conjugate: Horseradish peroxidase (HRP) labelled buffered matrix.
- Substrate Solution: Tetramethylbenzidine (TMB).
- Stop Solution: 2N hydrochloric acid solution.
- Wash Solution (10x Concentrated).
SENSITIVITY
The minimum sensitivity detection limit of cortisol using current cortisol ELISA kit was approximately 3.79 ng/ml. The dynamic range for this assay is 10.0 – 800.0 ng/ml.
ASSAY CHARACTERISTICS
– Analytical Sensitivity: 3.79 ng/ml
– Specificity (Cross Reactivity): Cortisol (100%), Prednisone (100%), Cortisone (76%), Prednisolone (54.3%), 11-Deoxycortisol (35.7%), Corticosterone (2.3%), 17-Hydroxyprogesterone (0.8%), 11-Deoxycorticosterone (0.5%), Estriol (0.4%), Pregnenolone, Estrone, Estradiol, DHEA, Testosterone, Danazole, Androstenedione, Dexamethasone, Progesterone (<0.1%).
– Assay Dynamic Range: 10 – 800 ng/ml
– Intra-Assay Variation: 6.3 – 8.0%
– Inter-Assay Variation: 4.2 – 6.4%
– Recovery: 91 – 99%
– Linearity: 100 – 113%
– Interfering Substances: None
– Drug Interferences: Any medication (cream, oil, pill, etc.) containing cortisol of course will significantly influence the measurement of this analyte.
REFERENCES
- Intranasal insulin attenuates the hypothalamic—pituitary—adrenal axis response to psychosocial stress. Psychoneuroendocrinology (2008) 33: 1394-1400. Andreas Bohringer A., et al.
- Thermoregulatory and endocrine responses to light pulses in short-day acclimated social voles (Microtus socialis). Chronobiology International (2007) 24 (2): 269-288. Zubidat AE, Ben-Shlomo R, Haim A.
- Effect of simultaneous versus apart administration of dexamethasone and alpha-ketoglutarate on growth hormone, cortisol and insulin-like growth factor-I in piglets. Bull Vet Inst Pulawy (2006) 50: 205-210. Sliwa E.
ADDITIONAL INFORMATION
- Full Name: Cortisol ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 3.79 ng/ml
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