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Cortisol ELISA Kit

INTENDED USE

Human cortisol ELISA kit is a reliable quantitative procedure for measuring cortisol in human serum and plasma. This assay has a minimum sensitivity detection of 3.79 ng/ml.

BACKGROUND

Cortisol (also called hydrocortisone and compound F) is a corticosteroid hormone or glucocorticoid that is produced in the zona fasciculate and the zona reticularis of the adrenal cortex. It is more commonly referred to as the “stress hormone” that is responsible for responding to stress. Over 90% of cortisol is found to be bound to cortisol-binding globulins (CBG) and around 7% is bound to albumin, only 1-3% is found unbound (this represent the active form of cortisol). Among the products that are released by the human adrenal cortex, only cortisol has been known to regulate ACTH secretion, for example the release of ACTH is inhibited through a negative feedback loop as the level of free cortisol (non-protein bound) in blood rises and the reverse happens when cortisol levels are subnormal.

The following cortisol ELISA kit is designed for using in various different types of diagnostic applications. The main biological effects of cortisol include deposition of liver glycogen, effect on fat metabolism, promotion of gluconeogenesis, anti-inflammatory action and the increase in blood glucose concentration when carbohydrate utilization has been reduced. Its measurement is a very useful indicator in diseases with abnormal glucocorticoid production e.g. Cushing Syndrome (hypercortisolism), Addison’s disease or even secondary adrenal insufficiency (hypocortisolism). Due to the diurnal fluctuation of cortisol levels it may be necessary to take several samples that are going to be used for an individual cortisol profile or used during dynamic tests such as ACTH stimulation and dexamethasone suppression tests.

CORTISOL ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Wells coated with anti-cortisol antibody.
  • Calibrators 0-5: Concentrations 0, 10, 30, 90, 270, 800 ng/ml.
  • Control 1 (Low)  And Control 2 (High): Containing cortisol in human serum.
  • Enzyme Conjugate: Horseradish peroxidase (HRP) labelled with cortisol in buffered matrix.
  • Substrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution: 2N hydrochloric acid solution.
  • Wash Solution (10x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of cortisol using current human cortisol ELISA kit was approximately 3.79 ng/ml. The dynamic range for this assay is 10.0 – 800.0 ng/ml.

ASSAY CHARACTERISTICS

– Cortisol: ELISA
– Analytical Sensitivity: 3.79 ng/ml
– Specificity (Cross Reactivity): Cortisol (100%), Prednisone (100%), Cortisone (76%), Prednisolone (54.3%), 11-Deoxycortisol (35.7%), Corticosterone (2.3%), 17-Hydroxyprogesterone (0.8%), 11-Deoxycorticosterone (0.5%), Estriol (0.4%), Pregnenolone, Estrone, Estradiol, DHEA, Testosterone, Danazole, Androstenedione, Dexamethasone, Progesterone (<0.1%).
– Assay Dynamic Range: 10 – 800 ng/ml
– Intra-Assay Variation: 6.3 – 8.0%
– Inter-Assay Variation: 4.2 – 6.4%
– Recovery: 91 – 99%
– Linearity: 100 – 113%
– Interfering Substances: None
– Drug Interferences: Any medication (cream, oil, pill, etc.) containing cortisol of course will significantly influence the measurement of this analyte.

REFERENCES

  1. Intranasal insulin attenuates the hypothalamic—pituitary—adrenal axis response to psychosocial stress. Psychoneuroendocrinology (2008) 33: 1394-1400. Andreas Bohringer A., et al.
  2. Thermoregulatory and endocrine responses to light pulses in short-day acclimated social voles (Microtus socialis). Chronobiology International (2007) 24 (2): 269-288. Zubidat AE, Ben-Shlomo R, Haim A.
  3. Effect of simultaneous versus apart administration of dexamethasone and alpha-ketoglutarate on growth hormone, cortisol and insulin-like growth factor-I in piglets. Bull Vet Inst Pulawy (2006) 50: 205-210. Sliwa E.

ADDITIONAL INFORMATION

  • Full Name: Cortisol ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 3.79 ng/ml

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