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EBV EA (Early Antigen) IgM ELISA Kit

INTENDED USE

Human EBV EA IgM ELISA kit is a procedure for analysing in-vitro quantitative levels of human IgM antibodies to the Epstein–Barr virus early antigen (EBV EA IgM) using samples of plasma or serum. This assay has a minimum analytical sensitivity limit of 1.1 U/ml.

BACKGROUND

Epstein–Barr virus (EBV) can also be referred to as human herpesvirus 4 (HHV-4) and it belongs to the family of viruses known as herpes. EBV is responsible for causing infectious mononucleosis, an illness which is associated with sore throat, fever, swollen lymph nodes and sometimes enlarged spleen. This virus is able to spread through many commonly bodily fluids, especially saliva. It is also able to spread through blood and semen, organ transplantations and blood transfusions. A large number of these viral infections have been linked to autoimmune diseases for example Sjögren’s syndrome, multiple sclerosis, dermatomyositis, systemic lupus erythematosus and rheumatoid arthritis. EBV Early antigen (EBV EA) can be associated to a number of different acute phase illness, however, after 3-6 months the levels become undetectable.

EBV EA IGM ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • EBV Early Antigen coated microtiter strips.
  • Calibrator B (Cut-Off Standard).
  • Calibrator D (Positive Control).
  • Calibrator A (Negative Control).
  • Calibrator C (Weak Positive Control).
  • Sample Diluent.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of IgM antibodies directed to Epstein–Barr virus early antigen (EBV EA-IgM) using current human EBV EA IgM ELISA kit was 1.1 U/ml. The dynamic range for this assay is 1.0 – 200.0 U/ml.

ASSAY CHARACTERISTICS

– EBV EA ELISA: IgM
– Intra-Assay-Precision: 6.2 %.
– Inter-Assay-Precision: 10.5 %.
– Inter-Lot-Precision: 2.4 – 11.1 %.
– Analytical Sensitivity: 1.10 U/mL.
– Recovery: 71 – 111 %.
– Linearity: 73 – 112 %.
– Cross-Reactivity: No cross-reactivity to Herpes, Measles, Mumps and Varicella.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity 85 %

REFERENCES

  1. IgM serum antibodies to Epstein-Barr virus are uniquely present in a subset of patients with the chronic fatigue syndrome. In Vivo. (2004) 18 (2): 101-6. Lerner A.M., et al.
  2. Antibodies to early EBV, CMV, and HHV6 antigens in systemic lupus erythematosus patients. Scand J Rheumatol. (2015) 44 (2): 143-9. Rasmussen N.S., et al.
  3. Epstein-Barr virus specific marker molecules for early diagnosis of infectious mononucleosis. J Virol Methods. (1988) 21 (1-4): 133-46. Middeldorp J.M., Herbrink P.
  4. EBV reactivation serological profile in primary Sjögren’s syndrome: an underlying trigger of active articular involvement? Rheumatol Int. (2013) 33 (5): 1149-57. Pasoto S.G., et al.
  5. Antibodies to Epstein-Barr virus-induced early antigens in blood donors. Acta Virol. (1989) 33 (4): 344-8. Wohlrabe P., et al.

ADDITIONAL INFORMATION

  • Full Name: EBV EA (Early Antigen) IgM ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.1 U/ml

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