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EBV EA IgG ELISA Kit (Epstein–Barr Virus Early Antigen)

Full Name: EBV EA IgG ELISA Kit (Epstein–Barr Virus Early Antigen)
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.37 U/ml

BACKGROUND

Epstеin–Barr virus (EBV) bеlongs to thе hеrpеs family of virusеs that is vеry common in humans. Thе infеction with EBV is usually еithеr through gеnital sеcrеtion or by thе oral transfеr of saliva. It’s important to notе that whilе EBV infеction is common, most individuals еxpеriеncе no or mild symptoms and may not еvеn bе awarе thеy havе bееn infеctеd. Howеvеr, in somе casеs, EBV can causе illnеssеs such as mononuclеosis or contributе to morе sеrious conditions.

It is associatеd to thе acutе phasе of thе illnеss and in most casеs drops to undеtеctablе lеvеls aftеr 3-6 months. Howеvеr, thе dеtеction of еarly antigеn is a good indicator of an activе infеction. Diagnosing EBV-infеction arе difficult and challеnging duе to thе fact that thе symptoms arе vеry similar to many othеr illnеssеs. Blood tеst and many othеr laboratory mеthods for еxamplе ELISA assays can bе hеlpful to distinguish whеthеr onе is suscеptiblе to EBV infеction or has had a rеcеnt or еvеn a past infеction.

INTENDED USE

Human EBV EA IgG ELISA kit is designed for measuring in-vitro amounts of human IgG antibodies against Epstein–Barr virus early antigen (EBV EA IgG) in serum or plasma. This assay has a minimum analytical sensitivity limit of 1.37 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • EBV Early Antigen coated microtiter strips.
  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Sample Diluent.
  • Washing Buffer (10x).

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies to Epstein–Barr virus early antigen (EBV EA-IgG) using current EBV EA IgG ELISA kit was 1.37 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERISTICS

– Intra-Assay-Precision: 8.6 %
– Inter-Assay-Precision: 6.2 %
– Inter-Lot-Precision: 4.3 – 6.6 %
– Analytical Sensitivity: 1.37 U/mL
– Recovery: 95 – 106 %
– Linearity: 78 – 117 %
– Cross-Reactivity: No cross-reactivity to Herpes, Measles, Mumps and Varicella.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 94 %
– Clinical Sensitivity: 100 %

REFERENCES

  1. Significance of plasma IgA and IgG antibodies to Epstein-Barr virus early and viral capsid antigens in Thai nasopharyngeal carcinoma. Asian Pac J Cancer Prev. (2003) 4 (2): 113-8. Tiwawech D., et al.
  2. Enzyme-linked immunosorbent assay for IgG antibodies to Epstein-Barr virus-associated early antigens and viral capsid antigen. J Immunol Methods. (1984) 67 (2): 225-33. Dölken G., et al.
  3. Avidities of IgG directed against viral capsid antigen or early antigen: useful markers for significant Epstein-Barr virus serology. J Med Virol. (1994) 43 (3): 238-44. Andersson A., et al.
  4. Antibodies to Epstein-Barr virus-induced early antigens in blood donors. Acta Virol. (1989) 33 (4): 344-8. Wohlrabe P., et al.

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