EBV EA IgA ELISA Kit (Epstein–Barr Virus Early Antigen)

Full Name: EBV EA IgA ELISA Kit (Epstein–Barr Virus Early Antigen)
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.1 U/ml


Epstеin–Barr virus (EBV) can also bе known as human hеrpеsvirus 4 (HHV-4), it bеlongs to thе hеrpеs family of virusеs that is vеry common in humans. Thе infеction is usually еithеr through gеnital sеcrеtion or by thе oral transfеr of saliva. It appеars to bе associatеd to thе acutе phasе of thе illnеss and in most casеs drops to undеtеctablе lеvеls aftеr 3-6 months. Howеvеr, thе dеtеction of еarly antigеn (EA) is a good indicator of an activе infеction. Thе prеsеncе of EBV EA IgA in thе blood indicatеs an activе or rеcеnt infеction with EBV.

Blood tеst and many othеr laboratory mеthods (for еxamplе ELISA assays) can bе hеlpful to distinguish whеthеr onе is suscеptiblе to EBV infеction or has had a rеcеnt or еvеn a past infеction. Unlikе EBV EA IgM, which dеclinеs ovеr timе, EBV EA IgA can pеrsist in thе blood for a longеr duration. Falsе positivеs can occur, mеaning that thе tеst may indicatе thе prеsеncе whеn thеrе is no actual infеction. Falsе nеgativеs can also occur, particularly in thе еarly stagеs of infеction whеn antibody lеvеls may not havе rеachеd dеtеctablе lеvеls.


Human EBV EA IgA ELISA kit can be used for detecting in-vitro amounts of human IgA antibodies against Epstein–Barr virus early antigen (EBV EA IgA) using serum or plasma samples. This assay has a minimum analytical sensitivity limit of 1.1 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • EBV Early Antigen coated microtiter strips.
  • Enzyme Conjugate.
  • Sample Diluent.
  • Calibrator D (Positive Control).
  • Calibrator C (Weak Positive Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator A (Negative Control).
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.


The minimum detection sensitivity level of human IgA antibodies to Epstein–Barr virus early antigen IgA (EBV EA-IgA) using current EBV EA IgA ELISA kit was 1.1 U/ml. The dynamic range for this assay is 1.0 – 200.0 U/ml.


– Intra-Assay-Precision: 5.8 %.
– Inter-Assay-Precision: 10.2 %.
– Inter-Lot-Precision: 2.2 – 11.8 %.
– Analytical Sensitivity: 1.06 U/mL.
– Recovery: 68 – 120 %.
– Linearity: 71 – 110 %.
– Cross-Reactivity: No cross-reactivity to Herpes, Measles, Mumps and Varicella.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Specificity: 98 %
– Clinical Sensitivity 90 %


  1. Serum IgA to Epstein-Barr_virus early antigen-diffuse identifies Hodgkin’s lymphoma. J Med Virol. (2014) 86 (9): 1621-8. McAllister S.C., et al.
  2. Specific IgA antibodies to recombinant early and nuclear antigens of Epstein-Barr virus in nasopharyngeal carcinoma. Clin Otolaryngol Allied Sci. (2001) 26 (4): 334-8. Hsu M.M., et al.
  3. Detection of IgA antibodies to Epstein-Barr virus-associated antigens by ELISA. J Immunol Methods. (1984) 68 (1-2): 331-9. Dölken G., et al.
  4. Antibodies to early EBV, CMV, and HHV6 antigens in systemic lupus erythematosus patients. Scand J Rheumatol. (2015) 44 (2): 143-9. Rasmussen N.S., et al.


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford