CCL22 ELISA Kit (MDC)

INTENDED USE

CCL22 ELISA kit is intended for detecting in vitro quantitative levels of CCL22 (MDC, macrophage-derived chemokine) in human tissue homogenates, plasma, serum and biological fluids. This assay has a minimum detection limit of 37.5 pg/ml.

BACKGROUND

Chemokine, cc motif, ligand 22 (CCL22) which can also be known as macrophage-derived chemokine (MDC) and small inducible cytokine subfamily A, member 22 (SCY22), is not particularly related to other chemokines. It only displays approx. 37% amino acid similarity with the thymus- and activation-regulated chemokine (TARC). The CCL22 gene is one of many different Cys-Cys (CC) cytokine genes clustered that are located on the q arm of chromosome 16. They display chemotactic activity for dendritic cells, chronically activated T lymphocytes, monocytes and natural killer cells.

CCL22 is a unique member of the CC chemokine family that has a fundamental role in the function of monocytes, dendritic cells and natural killer cells. The protein can elicits its effects on its target cells by interacting with cell surface chemokine receptors for example CCR4. The gene itself is located in the human chromosome 16 in a cluster along with other chemokines known as CCL17 and CX3CL1.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human CCL22 antibody.
• Standards: Concentrations 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml, made up from lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of MDC (macrophage-derived chemokine, CCL22) using current CCL22 ELISA kit was approximately 37.5 pg/ml. The dynamic range for this assay is 62.5 – 4,000 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CCL22, no cross reactivity or interference between CCL22 and analogues was detected.
– Recovery: Serum (85 – 98%), EDTA Plasma (85 – 105%), Heparin Plasma (85 – 103%).
– Linearity: Serum (90 – 102%), EDTA Plasma (88 – 92%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Macrophage-derived chemokine (MDC). J Leukoc Biol. (2000) 68 (3): 400-4. Review. Mantovani A., et al.
2. Chemokines and their receptors in the pathogenesis of allergic asthma: progress and perspective. Curr Opin Pulm Med. (2005) 11 (1): 35-42. Review. Bisset L.R. and Schmid-Grendelmeier P.
3. The CC chemokines MDC and TARC induce platelet activation via CCR4. Thromb Res. (2001) 101 (4): 279-89. Abi-Younes S., et al.
4. Regulation of macrophage-derived chemokine (MDC, CCL22) production. Crit Rev Immunol. (2002) 22 (2): 105-14. Review. Yamashita U. and Kuroda E.
5. Macrophage-derived chemokine (CCL22) is a novel mediator of lung inflammation following hemorrhage and resuscitation. Shock. (2014) 42 (6): 525-31. Richter J.R., et al.

ADDITIONAL INFORMATION

• Full Name: CCL22 ELISA Kit (MDC)
• Reactivity: Human
• Sample Type: Biological Fluids, Serum , Plasma, Tissue Homogenates
• Sensitivity: 62.5 pg/ml

OTHER RELATED ELISA KITS

• CCL17 ELISA Kit
• Cardiotrophin-1 ELISA Kit
• CXCL10 ELISA Kit