CCL22 ELISA kit is intended for detecting in vitro quantitative levels of CCL22 (MDC, macrophage-derived chemokine) in human tissue homogenates, plasma, serum and biological fluids. This assay has a minimum detection limit of 37.5 pg/ml.
Chemokine, cc motif, ligand 22 (CCL22) which can also be known as macrophage-derived chemokine (MDC) and small inducible cytokine subfamily A, member 22 (SCY22), is not particularly related to other chemokines. CCL22 only displays approx. 37% amino acid similarity with the thymus- and activation-regulated chemokine (TARC). The CCL22 gene is one of many different Cys-Cys (CC) cytokine genes clustered that are located on the q arm of chromosome 16.
These cytokines are a family of secreted proteins which are important for many different immuno-regulatory and inflammatory processes. They display chemotactic activity for dendritic cells, chronically activated T lymphocytes, monocytes and natural killer cells. There is also evidence of mild activity for primary activated T lymphocytes but it displays no chemoattractant activity for resting T lymphocytes, neutrophils and eosinophils.
Macrophage-derived chemokine (MDC) is however, highly expressed in monocyte-derived dendritic cells and macrophages but not in natural killer cells, monocytes and many cell lines of fibroblast, epithelial or endothelial origin. There was also high expression identified in normal thymus and less expression present in spleen and lung.
This suggests that MDC is a unique member of the CC chemokine family that has a fundamental role in the function of monocytes, dendritic cells and natural killer cells. The CCL22 protein can elicits its effects on its target cells by interacting with cell surface chemokine receptors for example CCR4. The gene itself is located in the human chromosome 16 in a cluster along with other chemokines known as CCL17 and CX3CL1.
CCL22 ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-human CCL22 antibody.
- Standards: Concentrations 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml, made up from lyophilized recombinant human MDC.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled CCL22 Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
The minimum detection sensitivity level of MDC (macrophage-derived chemokine, CCL22) using current human CCL22 ELISA kit was approximately 37.5 pg/ml. The dynamic range for this assay is 62.5 – 4,000 pg/ml.
– CCL22 (MDC): ELISA
– Specificity: Highly specific for CCL22, no cross reactivity or interference between CCL22 and analogues was detected.
– Recovery: Serum (85 – 98%), EDTA Plasma (85 – 105%), Heparin Plasma (85 – 103%).
– Linearity: Serum (90 – 102%), EDTA Plasma (88 – 92%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
- Macrophage-derived chemokine (MDC). J Leukoc Biol. (2000) 68 (3): 400-4. Review. Mantovani A., et al.
- Chemokines and their receptors in the pathogenesis of allergic asthma: progress and perspective. Curr Opin Pulm Med. (2005) 11 (1): 35-42. Review. Bisset L.R. and Schmid-Grendelmeier P.
- The CC chemokines MDC and TARC induce platelet activation via CCR4. Thromb Res. (2001) 101 (4): 279-89. Abi-Younes S., et al.
- Regulation of macrophage-derived chemokine (MDC, CCL22) production. Crit Rev Immunol. (2002) 22 (2): 105-14. Review. Yamashita U. and Kuroda E.
- Macrophage-derived chemokine (CCL22) is a novel mediator of lung inflammation following hemorrhage and resuscitation. Shock. (2014) 42 (6): 525-31. Richter J.R., et al.
- Full Name: CCL22 (MDC) ELISA Kit
- Reactivity: Human
- Sample Type: Bioloical Fluids, Serum , Plasma, Tissue Homogenates
- Sensitivity: 62.5 pg/ml
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