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Cardiotropin-1 (CT-1) ELISA Kit

INTENDED USE

Cardiotrophin-1 ELISA kit can be used for detecting in vitro quantitative levels of cardiotrophin-1 (CT-1) in human serum, plasma, biological fluids and tissue homogenates. This assay has a minimum detection limit of 9.375 pg/ml.

BACKGROUND

Cardiotrophin-1 (CT-1) is a cardiac hypertrophic factor of 21.5 kDa and is a member of the family of cytokines which includes interleukin-6, ciliary neurotrophic factor, interleukin-11, oncostatin M and leukemia inhibitory factor. Human CT-1 protein is made up of 201 amino acids and it shares approx. 80% amino acid identity with the 203-amino acid that is found in mouse CT-1 sequence. However, unlike the mouse protein, that human CT-1 contains 2 instead of 1 cys and also has no N-glycosylation site. Even though it lacks a signal sequence, the secreted CT-1 and mouse CT-1 still have the ability to induce cardiac myocyte hypertrophy in cell culture and also can bind to both mouse and human LIFR but not to OSMR.

CT-1 is primarily associated with many pathophysiology of heart diseases, for example congestive heart failure, myocardial infarction, valvular heart disease and hypertension. This protein can exert many cellular effects by interacting with leukemia inhibitory factor receptor beta (LIFR)/glycoprotein 130 (gp130) heterodimer.

Furthermore, a 1.7-kb CT-1 transcript has also been identified at high concentrations in heart, skeletal muscle, ovary and prostate. Low levels are detected in the kidney, thymus, pancreas, small intestine and testis. Little or no expression is seen in the brain, peripheral blood leukocytes, spleen, colon and placenta. Strong expression was observed in fetal kidney and lung. Finally, CT-1 can activate phosphatidylinositol 3-kinase (PI-3 kinase) activity in cardiac myocytes and also enhance the DNA -binding activities of the transcription factor NF-κB.

CARDIOTROPHIN-1 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human CT-1 antibody.
  • Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant human cardiotrophin-1.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled CT-1 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of CT-1 (cardiotrophin-1) using current human cardiotrophin-1 ELISA kit was approximately 9.375 pg/ml. The dynamic range for this assay is 15.625 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– Cardiotrophin-1 (CT-1): ELISA
– Specificity: Highly specific for CT-1, no cross reactivity or interference between CT-1 and analogues was detected.
– Recovery: Serum (87 – 102%), EDTA Plasma (85 – 103%), Heparin Plasma (85 – 100%).
– Linearity: Serum (87 – 103%), EDTA Plasma (85 – 100%), Heparin Plasma (81 – 90%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Cardiotrophin-1 (CT-1): a novel hypertrophic and cardioprotective agent. Int J Exp Pathol. (1999) 80 (4): 189-96. Review. Latchman D.S.
  2. Cardiotrophin-1 in cardiovascular regulation. Adv Clin Chem. (2010) 52: 41-76. Review. Jougasaki M.
  3. Vascular effects of glycoprotein130 ligands–part II: biomarkers and therapeutic targets. Vascul Pharmacol. (2012) 57 (1): 29-40. Review. Demyanets S., et al.
  4. Emerging evidence for the role of cardiotrophin-1 in cardiac repair in the infarcted heart. Cardiovasc Res. (2005) 65 (4): 782-92. Review. Freed D.H., et al.
  5. The Role of Leukemia Inhibitory Factor Receptor Signaling in Skeletal Muscle Growth, Injury and Disease. Adv Exp Med Biol. (2016) 900: 45-59. Review. Hunt L.C. and White J.
  6. Cardiotrophin-1: A multifaceted cytokine. Cytokine Growth Factor Rev. (2015) 26 (5):523-32. Review. López-Yoldi M., et al.

ADDITIONAL INFORMATION

  • Full Name: Cardiotrophin-1 (CT-1) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Tissue Homogenates, Serum, Other Biological Fluids
  • Sensitivity: 9.375 pg/ml

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