Bordetella Pertussis Toxin IgA ELISA Kit


Human Bordetella pertussis toxin IgA ELISA kit is intended for measuring quantitative and semi-quantitative levels of IgA class antibodies against Bordetella toxin (Bordetella Toxin-IgA) using plasma or serum samples. This assay has a minimum analytical sensitivity limit to a diagn. 97.3%.


Pertussis toxin (PT) is formed by Bordetella pertussis bacteria and it is a 105 kDa hexamer protein with a typical AB5 architecture. PT is fundamental in the colonisation of the respiratory tract and is also essential for the establishment of infection. It has also been shown to affect the innate immune response, by inhibiting early recruitment of macrophages and neutrophils and this can lead to interfering with chemokine production or inhibition of neutrophil chemotaxis. Recent publications have also suggested that PT may play an important therapeutic role in the treatment of many human ailments such as hypertension, autoimmune inhibition and viral inhibition.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).
  • Enzyme Conjugate.
  • TMB Substrate.
  • Sample Diluent.
  • Washing Buffer (10x).
  • Stop Solution.
  • Bordetella pertussis antigen coated microtiter strips.


The minimum detection sensitivity level of IgA class antibodies against Bordetella toxin (Bordetella Toxin-IgA) using current human Bordetella toxin IgA ELISA kit was to a diagn. 97.3%. The dynamic range for this assay was to a cut-off point.


  1. The concept of pertussis as a toxin-mediated disease. Pediatr Infect Dis. (1984) 3 (5): 467-86. Review. Pittman M.
  2. Development and validation of an Australian in-house anti-pertussis toxin IgG and IgA enzyme immunoassay. Pathology. (2013) 45 (2): 172-80. May M.L., et al.
  3. Serologic diagnosis of pertussis: evaluation of pertussis toxin and other antigens in enzyme-linked immunosorbent assay. J Infect Dis. (1990) 161 (5): 966-71. Mertsola J., et al.
  4. Adjuvant action of cholera toxin and pertussis toxin in the induction of IgA antibody response to orally administered antigen. Vaccine. (1993) 11 (2): 113-8. Wilson A.D., et al.
  5. Comparative role of immunoglobulin A in protective immunity against the Bordetellae. Infect Immun. (2007) 75 (9): 4416-22. Wolfe D.N., et al.


  • Full Name: Bordetella Pertussis Toxin IgA ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: diagn. 97.3%



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford