Bordetella Pertussis IgM ELISA Kit

Full Name: Bordetella Pertussis IgM ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.0 U/ml


Pertussis is usually described as an acute infectious disease which can be caused by the bacterium Bordetella pertussis. In order to create toxins that can paralyse the cilia and cause respiratory tract inflammation, the bacteria are able to connect to the cilia of the respiratory epithelial cells.

The toxins released by B. pertussis can paralyze the cilia, impairing their function in clearing mucus and foreign particles from the airways. This combination of factors results in the characteristic symptoms associated with pertussis, including severe coughing spells often accompanied by a “whooping” sound when inhaling. Understanding how B. pertussis interacts with respiratory epithelial cells helps shed light on why this infection can be particularly concerning for individuals with weakened immune systems or those who haven’t received proper vaccination against it.

It is a gram-negative, fastidious bacterium which can lead to the production of many biologically active and multiple antigenic products for example agglutinogens, tracheal cytotoxic, filamentous hemagglutinin (FHA), pertussis toxin, pertactin and adenylate cyclase. Additionally, it is a condition that exclusively affects people and is extremely contagious. People can spread it to one another by sneezing, coughing, or even by sharing breathing space while they are close to one another.


Human Bordetella pertussis IgM ELISA kit is designed for detecting in-vitro concentrations of human IgM antibodies to Bordetella pertussis (Bordetella-IgM) in serum and plasma. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Bordetella pertussis antigen coated microtiter strips.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Sample Diluent.
  • Washing Buffer (10x).
  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).


The minimum detection sensitivity level of human IgM antibodies against Bordetella pertussis (Bordetella-IgM) using current Bordetella pertussis IgM ELISA kit was 0.98 U/ml. The dynamic range for this assay is 1.0 – 75.0 IU/ml.


– Intra-Assay-Precision: 6.2 %
– Inter-Assay-Precision: 8.9 %
– Inter-Lot-Precision: 2.0 – 4.9 %
– Analytical Sensitivity: 1.0 U/mL
– Recovery: 107 – 123 %
– Linearity: 102 – 120 %
– Cross-Reactivity: No cross-reactivity to RSV, Adenovirus and Parainfluenza
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 100 %
– Clinical Sensitivity: 88 %


  1. High prevalence of antibody titers against Bordetella pertussis in an adult population with prolonged cough. Respir Med. (2008) 102 (11): 1586-91. Kapaskelis A.M., et al.
  2. A Novel IgM-capture enzyme-linked immunosorbent assay using recombinant Vag8 fusion protein for the accurate and early diagnosis of Bordetella pertussis-infection. Microbiol Immunol. (2016) 60 (5): 326-33. Otsuka N., et al.
  3. Evaluation of culture, immunofluorescence, and serology for the diagnosis of pertussis. J Clin Microbiol. (1989) 27 (4): 752-7. Halperin S.A., et al.
  4. The IgM and IgG response to Bordetella pertussis vaccination and infection. J Med Microbiol. (1981) 14 (1): 1-7. Macaulay M.E.
  5. Pertussis antibodies in the sera of children exposed to Bordetella-pertussis by vaccination or infection. J Hyg (Lond). (1973) 71 (1): 193-207. Dolby J.M. and Stephens S.


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