Bordetella Pertussis IgG ELISA Kit

Full Name: Bordetella Pertussis IgG ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 0.98 U/ml

BACKGROUND

Bordetella pertussis is a small coccobacillus that attaches to ciliated epithelial cells in the nasopharynx and trachea via adhesion proteins called fimbriae. Once attached, B. pertussis secretes toxins that damage the airway epithelium, impair immune responses, and cause pertussis symptoms. A key toxin is pertussis toxin, which inhibits G protein signaling in host cells leading to unrestrained cAMP production. This results in leukocyte dysfunction and inflammation. Another toxin, tracheal cytotoxin, disrupts the cilia on airway epithelial cells.

Pertussis disease has three stages: catarrhal, paroxysmal, and convalescent. It is highly contagious in the mild catarrhal stage before coughing begins. Severe coughing fits in the paroxysmal stage can cause exhaustion, apnea, and even death, especially in infants. Widespread vaccination has greatly reduced pertussis incidence but it remains a concern due to waning vaccine immunity. Adolescent and adult boosters and protecting vulnerable infants are important for control.

INTENDED USE

Human Bordetella pertussis IgG ELISA kit is a procedure for measuring in-vitro levels of human IgG antibodies to Bordetella pertussis (Bordetella-IgG) using either plasma or serum samples. This assay has a minimum analytical sensitivity limit of 0.98 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Bordetella pertussis antigen coated microtiter strips.
• Calibrator A (Negative Control).
• Calibrator B (Cut-Off Standard).
• Calibrator C (Weak Positive Control).
• Calibrator D (Positive Control).
• Enzyme Conjugate.
• TMB Substrate.
• Stop Solution.
• Sample Diluent.
• Washing Buffer (10x).

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies against Bordetella pertussis (Bordetella-IgG) using current Bordetella pertussis IgG ELISA kit was 0.98 U/ml. The dynamic range for this assay is 1.0 – 150.0 IU/ml.

ASSAY CHARACTERSTICS

– Intra-Assay-Precision: 5.0 %
– Inter-Assay-Precision: 4.3 %
– Inter-Lot-Precision: 2.6 – 4.5 %
– Analytical Sensitivity: 0.98 U/mL
– Recovery: 106 – 114 %
– Linearity: 78 – 124 %
– Cross-Reactivity: No cross-reactivity to RSV, Adenovirus and Parainfluenza IgG
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 84 %
– Clinical Sensitivity: 100 %

REFERENCES

1. Serum immunoglobulin G analysis to establish a delayed diagnosis of chronic cough due to Bordetella pertussis. Otolaryngol Head Neck Surg. (2012) 146 (1): 63-7. Bock J.M., et al.
2. Age-related differences in patterns of increased Bordetella pertussis-antibodies. Clin Vaccine Immunol. (2012) 19 (4): 545-50. Prince H.E., et al.
3. Antibody levels to Bordetella pertussis and Neisseria meningitidis in immunodeficient patients receiving immunoglobulin replacement therapy. J Clin Immunol. (2015) 35 (2): 213-7. Adam E. and Church J.A.
4. Different IgG-subclass distributions after whole-cell and acellular pertussis infant primary vaccinations in healthy and pertussis infected children. Vaccine. (2011) 29 (40): 6874-80. Hendrikx L.H., et al.
5. Does working in hospital increases seroprevalence and carrier state against Bordetella pertussis? Adv Biomed Res. (2015) 4: 194. Naeini A.E., et al.

OTHER RELATED ELISA KITS

• Bordetella IgM ELISA Kit
• Bordetella FHA IgG ELISA Kit
• Bordetella Toxin IgG ELISA Kit