Aspergillus Fumigatus IgM ELISA Kit

Full Name: Aspergillus Fumigatus IgM ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.04 U/ml

BACKGROUND

Aspergillus fumigatus has a filamentous structure consisting of a network of hyphae divided by septa. The conidiophores are specialized hyphae that give rise to asexual spores called conidia. Conidia are small (2-3 μm), unicellular, green structures that become airborne and allow dispersal of the fungus. Inhaled conidia that avoid elimination by the mucociliary escalator can swell and germinate inside alveoli, forming invasively growing hyphal masses.

Several virulence factors allow A. fumigatus to colonize and invade lung tissue. Thermotolerance allows survival at mammalian body temperature. Cell wall melanin helps resist host defenses and oxidative stress. Secreted proteases like Asp fumigatus protease cleave structural proteins in the extracellular matrix. Gliotoxin suppresses the immune system by inducing apoptosis in phagocytes. Biofilms formed by hyphae and extracellular matrix may confer resistance to antifungal drugs. Angioinvasion allows dissemination from lungs to other organs.

Aspergillus also causes disease through allergenic responses rather than infection. Bronchopulmonary aspergillosis occurs when colonization triggers a hypersensitivity immune reaction. Aspergillus can also cause asthma exacerbations by releasing conidia or fungal fragments that incite inflammation. Overall, the structure and virulence factors of A. fumigatus enable it to evade host defenses and establish disease in susceptible individuals.

INTENDED USE

Human Aspergillus fumigatus IgM ELISA kit is a method for in-vitro measurement of human IgM antibodies to Aspergillus fumigatus (Aspergillus fumigatus-IgM) using samples of plasma and serum. This assay has a minimum analytical sensitivity limit of 1.04 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Aspergillus fumigatus antigen coated microtiter strips.
• Enzyme Conjugate.
• TMB Substrate.
• Stop Solution.
• Sample Diluent.
• Washing Buffer (10x).
• Calibrator A (Negative Control).
• Calibrator B (Cut-Off Standard).
• Calibrator C (Weak Positive Control).
• Calibrator D (Positive Control).

SENSITIVITY

The minimum detection sensitivity level of human IgM antibodies against Aspergillus fumigatus using this Aspergillus fumigatus IgM ELISA kit was 1.04 U/ml. The dynamic range for this assay is 1.0 – 60.0 U/ml.

ASSAY CHARACTERSTICS

– Intra-Assay-Precision: 5.9 %
– Inter-Assay-Precision: 5.5 %
– Inter-Lot-Precision: 1.4 – 3.1 %
– Analytical Sensitivity: 1.04 U/mL
– Recovery: 90 – 104 %
– Linearity: 95 – 120 %
– Cross-Reactivity: No cross-reactivity to Candida albicans
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 99 %
– Clinical Sensitivity: 100 %

REFERENCES

1. A monoclonal IgM directed against immunodominant catalase B of cell wall of Aspergillus-fumigatus exerts anti-A. fumigatus activities. Mycoses. (2009) 52 (6): 524-33. Chaturvedi A.K., et al.
2. Studies on galactofuranose-containing glycostructures of the pathogenic mold Aspergillus fumigatus. Int J Med Microbiol. (2011) 301 (6): 523-30. Heesemann L., et al.
3. Immunologic observations in sera of a patient with allergic bronchopulmonary aspergillosis by means of the enzyme-linked immunosorbent assay. J Allergy Clin Immunol. (1984) 74 (5): 741-6. Kauffman H.F., et al.
4. Enzyme-linked immuno-filtration assay (ELIFA) for the detection of IgG, IgM, IgA and IgE antibodies against Aspergillus fumigatus. J Med Vet Mycol. (1987) 25 (2): 77-83. Pinon J.M., et al.

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• Aspergillus IgG ELISA Kit
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