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Echinococcus Granulosus IgG ELISA Kit

INTENDED USE

Human Echinococcus granulosus IgG ELISA kit is intended for measuring in-vitro quantitative concentrations of human IgG antibodies to Echinococcus granulosus (Echinococcus IgG) using serum or plasma samples. This assay has a minimum analytical sensitivity limit of 1.1 U/ml.

BACKGROUND

Echinococcus granulosus can also be called by many other names which includes hyper tape-worm, dog tapeworm or hydatid worm. It has the ability to infect many domestic and wild mammals in order to act as intermediate host for example humans, cats, sheep, horses, cattle, pigs, goats, kangaroos, camelids and deer. E. granulosus is found to be present around the world but the prevalence seems to vary considerably. It is often more abundant in rural areas which have livestock and wildlife in combination with poor sanitary conditions. Adult Echinococcus granulosus worms tend to be quite small (approx. 7mm), having only 4 segments where the final one is the largest and gravid (filled with eggs), the head (called scolex) consists of 4 suckers and there are a number of hooks which are useful for attaching to the gut wall.

ECHINOCOCCUS GRANULOSUS IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Echinococcus Coated Microplate (IgG).
  • Sample Diluent.
  • Stop Solution.
  • Washing Buffer (20x Conc.).
  • Echinococcus Anti-IgG Conjugate.
  • TMB Substrate Solution.
  • Positive Control.
  • Cut-off Control.
  • Negative Control.

SENSITIVITY

The minimum detection sensitivity level of IgG antibodies to Echinococcus granulosus (Echinococcus-IgG) using current human Echinococcus IgG ELISA kit was 1.1 U/ml. The dynamic range for this assay is 1.0 – 200.0 U/ml.

ASSAY CHARACTERISTICS

– Echinococcus Granulosus ELISA: IgG
– Cut-Off: 10 U
– Positive: > 11 U
– Equivocal: 9 – 11 U
– Negative: < 9 U
– Diagnostic Specificity: 98.82%
– Diagnostic Sensitivity: 97.22%
– Interferences: No interferences with hemolytic, lipemic or icteric samples was observed.
– Cross Reactivity: No cross reactivity was identified when a potentially cross-reacting sample panel was analysed.

REFERENCES

  1. Analysis of specific IgE and IgG subclass antibodies for diagnosis of Echinococcus granulosus. Parasite Immunol. (2006) 28 (8): 357-62. Khabiri A.R., et al.
  2. Microfluidic immunosensor with gold nanoparticle platform for the determination of immunoglobulin G anti-Echinococcus granulosus antibodies. Anal Biochem. (2011) 409 (1): 98-104. Pereira S.V., et al.
  3. Recombinant AgB8/1 ELISA test vs. commercially available IgG ELISA test in the diagnosis of cystic echinococcosis. Parasite Immunol. (2013) 35 (12): 433-40. Tamarozzi F., et al.
  4. Immunodiagnosis of human hydatid disease: Where do we stand? World J Methodol. (2015) 5 (4): 185-95. Review. Sarkari B. and Rezaei Z.

ADDITIONAL INFORMATION

  • Full Name: Echinococcus Granulosus IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.1 U/ml

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