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Aspergillus Fumigatus IgG ELISA Kit

Full Name: Aspergillus Fumigatus IgG ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.08 U/ml

BACKGROUND

In people with weakened immune systems, fumigatus can also act as an opportunistic pathogen, causing a disease called invasive aspergillosis. The structure of A. fumigatus consists of a network of filamentous hyphae divided by septa. Specialized hyphae known as conidiophores generate asexual spores called conidia, which enable dispersal of the fungus through the air. The tiny green conidia of A. fumigatus, measuring 2-3 μm, are unicellular and become airborne easily. When inhaled, conidia can swell and germinate inside the alveoli of the lungs, forming invasive masses of growing hyphae, if they are not eliminated by the mucociliary escalator.

Invasive growth of A. fumigatus hyphae in lung tissue is enabled by several virulence factors. Thermotolerance allows A. fumigatus to thrive at normal mammalian body temperature. The melanin in the cell wall helps the fungus resist host defenses and oxidative damage. Secreted proteases like Asp fumigatus protease can cleave structural proteins in the extracellular matrix. The toxin gliotoxin suppresses the immune system by triggering apoptosis in phagocytes. Biofilms created by hyphae and extracellular matrix may also make A. fumigatus resistant to antifungal medications. Angioinvasion permits dissemination of the fungus from the lungs to other bodily organs.

In addition to invasive infection, A. fumigatus can also provoke allergic disease. Colonization can lead to hypersensitivity reactions that cause bronchopulmonary aspergillosis. The release of conidia or fungal fragments by Aspergillus can exacerbate asthma through inflammatory effects.

INTENDED USE

Human Aspergillus fumigatus IgG ELISA kit is intended for in-vitro analysis of human IgG antibodies against Aspergillus fumigatus (Aspergillus fumigatus-IgG) in serum or plasma samples. This assay has a minimum analytical sensitivity limit of 1.08 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Aspergillus fumigatus antigen coated microtiter strips.
  • Calibrator A (Negative Control).
  • Calibrator B (Cut-Off Standard).
  • Calibrator C (Weak Positive Control).
  • Calibrator D (Positive Control).
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Sample Diluent.
  • Washing Buffer (10x).

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies t0 Aspergillus fumigatus using current Aspergillus fumigatus IgG ELISA kit was 1.08 U/ml. The dynamic range for this assay is 1.0 – 200.0 U/ml.

ASSAY CHARACTERSTICS

– Intra-Assay-Precision: 9.9 %
– Inter-Assay-Precision: 11.1 %
– Inter-Lot-Precision: 3.5 – 16.4 %
– Analytical Sensitivity: 1.08 U/mL
– Recovery: 87 – 97 %
– Linearity: 74 – 114 %
– Cross-Reactivity: No cross-reactivity to Candida albicans
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 81 %
– Clinical Sensitivity: 100 %

REFERENCES

  1. Allergic aspergillosis and the antigens of Aspergillus fumigatus. Curr Protein Pept Sci. (2014) 15 (5): 403-23. Review. Singh B., et al.
  2. Role of Aspergillus-fumigatus specific IgG in diagnosis and monitoring treatment response in allergic bronchopulmonary aspergillosis. Mycoses. (2017) 60 (1): 33-39. Agarwal R., et al.
  3. Precipitins and specific IgG antibody to Aspergillus fumigatus in a chest unit population. Thorax. (1992) 47 (1): 48-52. Faux J.A., et al.
  4. The uptake of Aspergillus fumigatus-protein by serum IgG antibody from patients with pulmonary aspergillosis. Clin Allergy. (1977) 7 (2): 117-25. Jacoby B., et al.

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