Rat Angiotensinogen Intact ELISA Kit (AGT)

Full Name: Rat Angiotensinogen Intact ELISA Kit (AGT)
Reactivity: Rat
Sample Type: Cell Culture Supernatant, Urine, Serum, Plasma,
Sensitivity: 0.02 ng/ml

BACKGROUND

An a-2-globulin generated by the liver, angiotensinogen (AGT) is a member of the serpin protein family. It controls fluid balance and blood pressure (renin-angiotensin system). It is a significant protein that is a member of the serpin protein family. Controlling these processes aids in preserving the body’s normal equilibrium.

In the body, AGT is involved in a number of important processes. This system helps maintain homeostasis with its control of blood pressure and fluid balance. Through their control over the synthesis of ADH, the kidneys play a significant part in this system and are in charge of controlling intracellular fluid content. When ADH levels are high, they increase the permeability of small intestinal cells to water allowing more water to be taken into the body’s cells which increases osmolarity. The kidneys then act to decrease ADH levels and restore the balance, returning osmolarity to normal. When it is felt that more water would be beneficial for survival, signals are sent from the brain stem, hypothalamus and amygdala to activate receptors in other parts of the body which signal thirst.

In addition to these physiological mechanisms, another way in which humans monitor their hydration levels is through perception of thirst or by means of behavioural adaptations such as drinking a glass of water when they feel thirsty or experiencing a sensation associated with dehydration. The path to our perception of thirst may be long, but the sensation leads to a reflex that prompts us to drink, which we might have done thousands of times without thinking about it.

Angiotensinogen is made in the liver and adrenal glands, with pregnant women producing the most of it. The renin-angiotensin-aldosterone system (RAAS) initiates it and this raises blood pressure levels. In addition to being present in many drugs, the RAAS is also connected to the emergence of various disorders. When blood pressure levels increase, the body releases this enzyme which converts larger molecules into smaller ones, raising blood pressure and creating an environment conducive to fluid retention.

INTENDED USE

Rat angiotensinogen intact ELISA kit is a method for quantitative detection of intact angiotensinogen (AGT) in rat serum, cell culture supernatant, EDTA-plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.02 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Pre-Coated Plate: Anti-Rat AGT (AI).
Labeled Antibody (30x Conc.).
Standard: Recombinant.
EIA Buffer.
Solution For Labeled Antibody.
TMB Chromogen.
Stop Solution.
Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of angiotensinogen (AGT intact) using current rat angiotensinogen intact ELISA kit was approximately 0.02 ng/ml. The dynamic range for this assay is 0.08 – 5.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.02 ng/mL
– Measurement Range: 0.08 – 5 ng/mL
– Added Recovery Assay: 86.2 – 94.2%
– Intra – Assay: 4.1 – 5.0%
– Inter – Assay: 4.3 – 5.2%
– Specificity: 100%

REFERENCES

Distribution of immunoreactive angiotensin II, angiotensin I, angiotensinogen and renin in the central nervous system of intact and nephrectomized rats. Hypertension. (1984) 6 (2 Pt 2): I130-6. Healy D.P. and Printz M.P.
Reduced hypertension-induced end-organ damage in mice lacking cardiac and renal angiotensinogen synthesis. J Mol Med (Berl). (2002) 80 (6): 359-66. Kang N., et al.
An intact renin-angiotensin system is a prerequisite for normal renal development. J Hypertens. (2000) 18 (2): 123-37. Review. Guron G. and Friberg P.
Effect of angiotensin II on angiotensinogen_production in adrenalectomized rats. Life Sci. (1990) 46 (23): 1657-60. Nakamura A., et al.
Hormonal control of angiotensinogen production. Life Sci. (1982) 30 (7-8): 577-84. Dzau V.J. and Herrmann H.C.
Sexual dimorphism of urine angiotensinogen-excretion in the rat. Jpn J Pharmacol. (1994) 64 (4): 243-50. Wang E., et al.

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