ACTH ELISA Kit (Adrenocorticotropic Hormone)
INTENDED USE
Human ACTH ELISA kit is a reliable quantitative procedure for measuring adrenocorticotropic hormone (ACTH) in human plasma (EDTA) samples. This assay has a minimum sensitivity detection limit of 0.22 pg/ml.
BACKGROUND
Adrenocorticotropic hormone (ACTH), a corticotropin with a molecular weight of roughly 4500 Dalton, is a peptide hormone made up of 39 amino acids. In reaction to corticotropin releasing hormone, it is secreted by corticotropes in the adenohypophysis (anterior lobe) of the pituitary gland. It is a hormone that promotes the transformation of fuel that has been saved into energy.
The main consequence of this in humans is a rise in blood sugar levels brought on by an increase in the liver’s gluconeogenesis. Aside from increasing adrenal medulla activity, the production of ACTH also affects other metabolic processes like; protein synthesis, lipid metabolism, and amino acid metabolism.
The hypothalamus pituitary adrenal axis and ACTH have a feedback loop that stimulates the adrenal cortex’s release of glucocorticoids. When cortisol levels are insufficient or glucocorticoids are required for stress, its levels rise. By releasing glucose from the liver, speeding up the liver’s conversion of pyruvate to acetaldehyde, activating lipase, and releasing lipids from adipose tissue, adrenocorticotropic hormone contributes significantly to a number of metabolic processes. This promotes homeostasis and guarantees the body’s correct operation.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Reagent 1: Biotinylated affinity purified goat antibody.
- Reagent 2: Enzyme peroxidase labelled mouse monoclonal.
- Wash Solution: Concentrate (Saline with surfactant).
- Substrate: Tetramethylbenzidine (TMB).
- Stopping solution: 1 N sulfuric acid.
- Microplate (12 x 8-well strips): One holder containing adrenocorticotropic hormone antigen coated wells.
- Calibrators A-F: Concentration 0-500 pg/ml.
- Zero Calibrator: Lyophilized synthetic.
- Controls 1 And Control 2: Containing synthetic h-ACTH (1-39).
SENSITIVITY
The minimum sensitivity detection limit of adrenocorticotropic hormone (ACTH) using current ACTH ELISA kit was approximately 0.22 pg/ml. The dynamic range for this assay is 7.2 – 564.0 pg/ml.
ASSAY CHARACTERISTICS
– Intra-Assay Variation: 2.27 – 6.71%
– Inter-Assay Variation: 6.9 – 7.1%
– Recovery: 87 – 103%
– Linearity: 89 – 122%
REFERENCES
- Expression of adrenocorticotropic hormone, prolactin and transcriptional factors in clinically nonfunctioning pituitary adenoma. Endocr J. (2007) 54 (6): 961-8. Kageyama K., et al.
- An analysis of circadian rhythms in human adrenocortical secretory activity. Trans Am Clin Climatol Assoc. (1966) 77: 151-60. Liddle GW.
- Circadian cortisol secretory rhythms in Cushing’s disease. J Clin Endocrinol Metab. (1979) 48 (5): 760-5. Boyar R.M., et al.
- Circadian rhythms and levels of beta-endorphin, ACTH, and cortisol during chronic methadone maintenance treatment in humans. Life Sci. (1983) 33 Suppl 1: 409-11. Kreek M.J., et al.
ADDITIONAL INFORMATION
- Full Name: ACTH ELISA Kit (Adrenocorticotropic Hormone)
- Reactivity: Human
- Sample Type: Plasma (EDTA)
- Sensitivity: 0.22 pg/ml
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