Adrenaline Research ELISA Kit (Epinephrine)

INTENDED USE

Adrenaline research ELISA kit can be used for accurately measuring in vitro quantitative levels of epinephrine (adrenaline) in human EDTA-plasma, cell culture and many other biological samples types. This assay has a minimum sensitivity detection limit of 0.25 ng/ml.

BACKGROUND

Epinephrine (adrenaline), can be referred to as a catecholamine since it contains a catechol moiety. It is strong emotions such as anger and fear which cause adrenaline to be released into the bloodstream, this leads to an; increase in blood pressure, heart rate, muscle strangle and sugar metabolism. It is way the body is preparing its self for strenuous activity, this process is often known as the “flight or fight response”.

Adrenaline can cause the smooth muscles which are contained in the lungs to relax by binding to beta-2-adrenergic receptors on bronchiole muscle cells, this enables intensified respiration by causing the bronchioles to relax. Another role is to have an effect in the liver, together with glucagon, adrenaline is able to break down glycogen energy stores. Finally in the heart muscles cells epinephrine binds beta-adrenergic receptors which results in the hearts contraction rate, leading to increased levels of blood being supplied to all the tissues in the body.

This is a flexible competitive assay system which be used for many different biological sample types and volumes. The sample is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: 1 x 96 wells (empty).
• Adhesive Foil: Adhesive Foils in a re-sealable pouch.
• Wash Buffer Concentrate (50x).
• Enzyme Conjugate.
• Substrate: TMB Chromogenic.
• Stop Solution.
• Adrenaline Microtiter Strips: Antigen pre-coated microwell plate.
• Antiserum: Rabbit anti-adrenaline antibody.
• Adjustment Buffer.
• Standards (A-F): Concentrations 0, 0.45, 1.5, 4.5, 15, 45 ng/ml.
• Controls (1+2).
• Acylation Buffer.
• Acylation Reagent.
• Coenzyme: S-adenosyl-L-methionine.
• Enzyme (Lyophilized): Catechol-O-methyltransferase.
• Extraction Plate: 2 x 48 well plates coated with boronate affinity gel.
• Hydrochloric Acid.

SENSITIVITY

The minimum sensitivity detection limit of epinephrine or adrenaline using current epinephrine research ELISA kit was approximately 0.25 ng/ml. The dynamic range for this assay is 0.5 – 80 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity Adrenaline (Limit of Detection): 0.3 ng/mL x C* (C* = Correction factor).
– Intra Assay Precision: 9.3 – 17.1%
– Inter Assay Precision: 11.2 – 16.3%
– Recovery: 89.4 – 128.3% (EDTA-Plasma) and 81.6 – 109.6% (Cell Culture Medium).

REFERENCES

1. Effect of circulating epinephrine on platelet function and hematocrit. Hypertension. (1995) 25 (5): 1096-105. Kjeldsen S.E., et al.
2. Enzyme-linked immunoassay for plasma-free metanephrines in the biochemical diagnosis of phaeochromocytoma in adults is not ideal. Clin Chem Lab Med. (2011) 50 (1): 105-10. Mullins F., et al.
3. An update on self-injectable epinephrine. Curr Opin Allergy Clin Immunol. (2013) 13 (4): 432-7. Review. Rudders S.A. and Banerji A.
4. Epinephrine and the metabolic syndrome. Curr Hypertens Rep. (2012) 14 (1): 1-7. Review. Ziegler M.G., et al.

ADDITIONAL INFORMATION

• Full Name: Adrenaline Research ELISA Kit (Epinephrine)
• Reactivity: Human
• Sample Type: Plasma (EDTA), Cell Culture, Other Biological Samples
• Sensitivity:  0.25 ng/ml

OTHER RELATED ELISA KITS

• Noradrenaline (Norepinerphrine) Research ELISA Kit
• 5-HIAA ELISA Kit
• Dopamine Research ELISA Kit