Human noradrenaline research ELISA kit can be used for detecting in vitro quantitative concentrations of noradrenaline and norepinephrine in human plasma, other biological samples and cell culture medium. This assay has a minimum detection limit of 1.3 pg/ml.
Noradrenaline (also known as norepinephrine) mainly functions in the brain and it is a member of the catecholamines family. Outside of the brain, one of its primary roles again is of a neurotransmitter through the sympathetic ganglia or it can be directly into the blood steam through the adrenal glands. It requires binding to specific surface receptors in order to have an effect of certain target cells, this is regardless of how and where it is being released. It is at its lowest during sleep and can reach peak levels during situations of stress and danger, this is often referred to as a fight-or-flight response.
In the brain, noradrenaline can be formed in the nuclei or within closely packed brain cell neurons. It has a predominate role in mobilising the brain for efficient functioning, where it is widely known in helping to increase alertness and arousal, increase anxiety and restlessness, enhance the retrieval and formation of memory, promoting vigilance and focus attention.
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: 1 x 96 wells (empty).
- Adhesive Foil.
- Wash Buffer Concentrate (50x).
- Enzyme Conjugate.
- TMB Substrate.
- Stop Solution.
- Noradrenaline Coated Microtiter Strips.
- Noradrenaline Antiserum.
- Adjustment Buffer.
- TE Buffer.
- Standards (A-F): Concentrations 0. 0.2, 0.6, 2, 8, 32 ng/ml
- Controls (1+2).
- Acylation Buffer.
- Acylation Reagent.
- Enzyme (Lyophilized).
- Extraction Plate.
- Hydrochloric Acid.
The minimum sensitivity level of norepinephrine or noradrenaline using current noradrenaline research ELISA kit was approximately 1.3 pg/ml. The dynamic range for this assay is 0.2 – 32 ng/ml.
– Analytical Sensitivity: 1.3 pg/ml
– Functional Sensitivity: 2 pg/ml
– Analytical Sensitivity (Cross Reactivity): Derivatized Noradrenaline (100%)
– Intra Assay Precision (EDTA-Plasma): 8.4 – 15.6%
– Inter Assay Precision (Cell Culture Media, RPMI): 8.4 – 12.2%
– Recovery: 6.9% (EDTA-Plasma) and 17.7% (RPMI)
- Effects of hypocretin and norepinephrine-interaction in bed nucleus of the stria terminalis on arterial pressure. Neuroscience. (2013) 255: 278-91. Ciriello J., et al.
- Norepinephrine: a neuromodulator that boosts the function of multiple cell types to optimize CNS performance. Neurochem Res. (2012) 37 (11): 2496-512. Review. O’Donnell J., et al.
- Altered locus coeruleus-norepinephrine function following single prolonged stress. Eur J Neurosci. (2013) 37 (6): 901-9. George S.A., et al.
- Regulation of the norepinephrine-transporter by endothelins: a potential therapeutic target. Vitam Horm. (2015) 98: 371-405. Review. Vatta M.S., et al.
- Full Name: Noradrenaline Research ELISA Kit (Norepinephrine)
- Reactivity: Human
- Sample Type: Plasma, Other Biological Samples, Cell Culture Medium
- Sensitivity: 1.3 pg/ml
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