Dopamine Research ELISA Kit (Hydroxytyramine)

INTENDED USE

Dopamine research ELISA kit is intended for detecting in vitro quantitative levels of dopamine (hydroxytyramine) in cell culture, plasma (EDTA) and many other biological samples. This assay has a minimum sensitivity detection limit of 0.25 ng/ml.

BACKGROUND

Dihydroxyphenylalanine is converted into the chemical molecule dopamine (hydroxytyramine), which contains nitrogen. It functions as a neurotransmitter in the control of motivation, drive and pleasure. L-DOPA decarboxylase and aromatic amino acid decarboxylase enzymes, which create dopamine from L-dopa and tyrosine, respectively, are the main sources of synthesis in humans. Presynaptic neurons discharge it into the gap junction. Through a process known as exocytosis, signals are sent from one neuron to another neuron through the tiny space between two nerve cells.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate – 1 x 96 wells (empty).
• Adhesive Foil.
• Wash Buffer Concentrate (50x).
• Enzyme Conjugate.
• TMB Substrate.
• Stop Solution.
• Dopamine Microtiter Strips: Antigen pre-coated microwell plate.
• Antiserum – Rabbit anti-dopamine antibody.
• Adjustment Buffer.
• Standards (A-F): Concentrations 0, 0.9, 3, 9, 30, 90 ng/ml.
• Controls 1+ Control 2.
• Hydrochloric Acid.
• Acylation Concentrate.
• Acylation Buffer.
• Acylation Diluent.
• Assay Buffer.
• Coenzyme: S-adenosyl-L-methionine.
• Enzyme (Lyophilized): Catechol-O-methyltransferase (COMT).
• Extraction Plate (2x 48 well plates).
• Extraction Buffer.

SENSITIVITY

The minimum sensitivity detection limit of hydroxytyramine (dopamine) using current dopamine research ELISA kit was approximately 0.25 ng/ml. The dynamic range for this ELISA assay is 0 – 80 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity (Limit of Detection): 0.7 ng/mL x C* (C* = Correction factor).
– Intra Assay Precision: 21.5 – 10.0% (EDTA-Plasma)
– Inter Assay Precision: 14.8 – 23.0% (Cell Culture Medium/RPMI).
– Recovery: 83.7 – 115.9% (EDTA-Plasma) and 77.7 – 113.4% (Cell Culture Medium/RPMI).

REFERENCES

1. Safety out of control:dopamine and defence. Behav Brain Funct. (2016) 12 (1): 15. Review. Lloyd K. and Dayan P.
2. Dopamine-signals and physiological origin of cognitive dysfunction in Parkinson’s disease. Mov Disord. (2015) 30 (4): 472-83. Review. Matsumoto M.
3. Striatal dopamine,reward, and decision making in schizophrenia. Dialogues Clin Neurosci. (2016) 18 (1): 77-89. Review. Deserno L., et al.
4. Protective and toxic roles of dopamine in Parkinson’s disease. J Neurochem. (2014) 129 (6): 898-915. Review. Segura-Aguilar J., et al.

ADDITIONAL INFORMATION

• Full Name: Dopamine Research ELISA Kit (Hydroxytyramine)
• Reactivity: Any Species
• Sample Type: Biological Samples, Cell Culture, Plasma (EDTA)
• Sensitivity: 0.25 ng/ml

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