Dopamine research ELISA kit is intended for detecting in vitro quantitative levels of dopamine (hydroxytyramine) in cell culture, plasma (EDTA) and many other biological samples. This assay has a minimum sensitivity detection limit of 0.25 ng/ml.
Dihydroxyphenylalanine is converted into the chemical molecule dopamine (hydroxytyramine), which contains nitrogen. It functions as a neurotransmitter in the control of motivation, drive and pleasure. L-DOPA decarboxylase and aromatic amino acid decarboxylase enzymes, which create dopamine from L-dopa and tyrosine, respectively, are the main sources of synthesis in humans. Presynaptic neurons discharge it into the gap junction. Through a process known as exocytosis, signals are sent from one neuron to another neuron through the tiny space between two nerve cells.
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate – 1 x 96 wells (empty).
- Adhesive Foil.
- Wash Buffer Concentrate (50x).
- Enzyme Conjugate.
- TMB Substrate.
- Stop Solution.
- Dopamine Microtiter Strips: Antigen pre-coated microwell plate.
- Antiserum – Rabbit anti-dopamine antibody.
- Adjustment Buffer.
- Standards (A-F): Concentrations 0, 0.9, 3, 9, 30, 90 ng/ml.
- Controls 1+ Control 2.
- Hydrochloric Acid.
- Acylation Concentrate.
- Acylation Buffer.
- Acylation Diluent.
- Assay Buffer.
- Coenzyme: S-adenosyl-L-methionine.
- Enzyme (Lyophilized): Catechol-O-methyltransferase (COMT).
- Extraction Plate (2x 48 well plates).
- Extraction Buffer.
The minimum sensitivity detection limit of hydroxytyramine (dopamine) using current dopamine research ELISA kit was approximately 0.25 ng/ml. The dynamic range for this ELISA assay is 0 – 80 ng/ml.
– Analytical Sensitivity (Limit of Detection): 0.7 ng/mL x C* (C* = Correction factor).
– Intra Assay Precision: 21.5 – 10.0% (EDTA-Plasma)
– Inter Assay Precision: 14.8 – 23.0% (Cell Culture Medium/RPMI).
– Recovery: 83.7 – 115.9% (EDTA-Plasma) and 77.7 – 113.4% (Cell Culture Medium/RPMI).
- Safety out of control:dopamine and defence. Behav Brain Funct. (2016) 12 (1): 15. Review. Lloyd K. and Dayan P.
- Dopamine-signals and physiological origin of cognitive dysfunction in Parkinson’s disease. Mov Disord. (2015) 30 (4): 472-83. Review. Matsumoto M.
- Striatal dopamine,reward, and decision making in schizophrenia. Dialogues Clin Neurosci. (2016) 18 (1): 77-89. Review. Deserno L., et al.
- Protective and toxic roles of dopamine in Parkinson’s disease. J Neurochem. (2014) 129 (6): 898-915. Review. Segura-Aguilar J., et al.
- Full Name: Dopamine Research ELISA Kit (Hydroxytyramine)
- Reactivity: Any Species
- Sample Type: Biological Samples, Cell Culture, Plasma (EDTA)
- Sensitivity: 0.25 ng/ml
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