Dopamine research ELISA kit is intended for detecting in vitro quantitative levels of dopamine (hydroxytyramine) in cell culture, plasma (EDTA) and many other biological samples. This assay has a minimum sensitivity detection limit of 0.25 ng/ml.
Dopamine (hydroxytyramine) is a nitrogen containing organic compound which is produced as an intermediate compound from dihydroxyphenylalanine (dopa). It is one of the catecholamine neurotransmitters in the brain, which can be derived from tyrosine and is a precursor to adrenalin (epinephrine) and noradrenalin (norepinephrine). Dopamine has a major function as a neurotransmitter where it has the ability to inhibit the transmission of nerve impulses in basal ganglia, substantia nigra and the corpus striatum of the brain. There are a family of dopamine receptors which are responsible for mediating its action, where dopamine binds alpha-1 and beta-1 adrenergic receptors.
Dopamine (hydroxytyramine) is a chemical in your brain that is responsible for affecting your emotions, movements and your sensations of pleasure and pain. It is a neurotransmitter which is located in the deep middle region of the brain which is known as the substantia nigra. A deficiency of dopamine is linked with cellular death in the substantia nigra which can lead to Parkinson disease. Even any abnormalities in dopamine transmission, for example hyperactive dopamine transmission in certain parts of the brain, have been associated with psychotic syndromes (e.g. schizophrenia). However, dopaminergic structures within the brain (mainly the nucleus accumbens and the striatum) have been implicated in reward related behaviour.
The following dopamine research ELISA kit is based on a competitive procedure, due to the fact that the chemical structure of catecholamines is identical in all animals and the performance of a pre-extraction step means that this assay is able to be used for a wide range of animal species. Dopamine has been extracted using a cis-diol-specific affinity gel, acylated and then enzymatically derivatized.
DOPAMINE RESEARCH ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate – 1 x 96 wells (empty).
- Adhesive Foil.
- Wash Buffer Concentrate (50x).
- Enzyme Conjugate.
- TMB Substrate.
- Stop Solution.
- Dopamine Microtiter Strips: Antigen pre-coated microwell plate.
- Antiserum – Rabbit anti-dopamine antibody.
- Adjustment Buffer.
- Standards (A-F): Concentrations 0, 0.9, 3, 9, 30, 90 ng/ml.
- Controls 1+ Control 2.
- Hydrochloric Acid.
- Acylation Concentrate.
- Acylation Buffer.
- Acylation Diluent.
- Assay Buffer.
- Coenzyme: S-adenosyl-L-methionine.
- Enzyme (Lyophilized): Catechol-O-methyltransferase (COMT).
- Extraction Plate (2x 48 well plates).
- Extraction Buffer.
The minimum sensitivity detection limit of hydroxytyramine (dopamine) using current dopamine research ELISA kit was approximately 0.25 ng/ml. The dynamic range for this ELISA assay is 0 – 80 ng/ml.
– Dopamine Research: ELISA
– Analytical Sensitivity (Limit of Detection): 0.7 ng/mL x C* (C* = Correction factor).
– Intra Assay Precision: 21.5 – 10.0% (EDTA-Plasma)
– Inter Assay Precision: 14.8 – 23.0% (Cell Culture Medium/RPMI).
– Recovery: 83.7 – 115.9% (EDTA-Plasma) and 77.7 – 113.4% (Cell Culture Medium/RPMI).
- Safety out of control: dopamine and defence. Behav Brain Funct. (2016) 12 (1): 15. Review. Lloyd K. and Dayan P.
- Dopamine signals and physiological origin of cognitive dysfunction in Parkinson’s disease. Mov Disord. (2015) 30 (4): 472-83. Review. Matsumoto M.
- Striatal dopamine, reward, and decision making in schizophrenia. Dialogues Clin Neurosci. (2016) 18 (1): 77-89. Review. Deserno L., et al.
- The Role of Dopamine and Its Dysfunction as a Consequence of Oxidative Stress. Oxid Med Cell Longev. (2016) 2016: 9730467. Review. Juárez Olguín H., et al.
- Protective and toxic roles of dopamine in Parkinson’s disease. J Neurochem. (2014) 129 (6): 898-915. Review. Segura-Aguilar J., et al.
- Full Name: Dopamine Research ELISA Kit
- Reactivity: Any Species
- Sample Type: Biological Samples, Cell Culture, Plasma (EDTA)
- Sensitivity: 0.25 ng/ml
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