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Rat Adiponectin ELISA Kit (ADP)

Full Name: Rat Adiponectin ELISA Kit (ADP)
Reactivity: Rat
Sample Type: Plasma, Serum
Sensitivity:  < 0.081 ng/ml

BACKGROUND

Adiponectin (ADP) is expressed within adipose tissues, the main functions involves increasing insulin resistance. This is demonstrated by a decrease in the levels present in plasma for individuals with coronary artery disease, obesity and type 2 diabetes. Adiponectin is not just found in humans but also in rodents. and non-human primates. This hormone is produced from the adipocyte protein and released into the bloodstream. In addition to improving insulin sensitivity, it directly reduces inflammation. ADP also increases glucose uptake which helps lessen hyperglycemia. However this does not lead to an increase in insulin levels so fat cells can store energy as fat rather than glycogen. Its been proven to have anti-inflammatory effects when present  in large amounts.

An N-terminal collagen domain and a globular domain (C-terminal) are both present in the 244 amino acids that make up adiponectin. The N-terminal is found to be conserved across species and plays an important role in multimer formation. It circulates in the blood as multiple isoforms, which play critical roles in influencing the inflammatory process during chronic and acute phase diseases.

INTENDED USE

Rat adiponectin ELISA kit can be used for determining quantitative amounts of ADP (adiponectin) in rat serum or plasma samples. This assay has a minimum sensitivity detection limit of < 0.081 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: coated with anti-rat Adiponectin antibody.
  • Standards A-F: Lyophilised, contain native rat adiponectin. Standard values are between 0.25, 0.75, 1.5, 3, 6.5 und 10 ng/ml.
  • Control Serum KS1 & KS2.
  • Dilution Buffer.
  • Antibody-POD-Conjugate.
  • Substrate.
  • Washing Buffer (20x).
  • Stopping Solution.
  • Sealing Tape (2x Adhesive).

SENSITIVITY

The minimum sensitivity detection limit of ADP (adiponectin) using current rat adiponectin ELISA kit was approximately 0.081 ng/ml. The dynamic range for this assay is 0.25 – 10.0 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.081 ng/mL.
– Inter Assay Variability: 1.62 – 11.23 µg/mL (mean).
– Intra Assay Variability: 5.709 – 12.702 µg/mL (mean).

REFERENCES

  1. Changes in adiponectin expression in acute myocardial infarction rats and the significance of bisoprolol intervention. Can J Physiol Pharmacol. (2011) 89 (2): 109-15. Zhang S., et al.
  2. Effects of fasting and refeeding on serum leptin, adiponectin and free fatty acid concentrations in young and old male rats. Gerontology. (2005) 51 (6): 357-62. Kmiec Z., et al.
  3. Protective role of adiponectin in a rat model of intestinal ischemia reperfusion injury. World J Gastroenterol. (2015) 21 (47): 13250-8. Liu X.H., et al.
  4. Adiponectin is associated with serum and adipose tissue fatty acid composition in rats. J Endocrinol Invest. (2009) 32 (8): 659-65. Pérez de Heredia F., et al.
  5. Possible implications of leptin, adiponectin and ghrelin in the regulation of energy homeostasis by thyroid hormone. Endocrine. (2007) 32 (1): 30-2. Kokkinos A., et al.

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