Mouse Adiponectin ELISA Kit (Acrp30)

Full Name: Mouse Adiponectin ELISA Kit (Acrp30)
Reactivity: Mouse
Sample Type: Plasma, Serum
Sensitivity: < 0.008 ng/ml

BACKGROUND

Adiponectin (also known as Acrp30, AdipoQ, GBP-28 and apM1) is a protein that is involved in regulating glucose and fatty acids breakdown. It is a protein hormone which modulates metabolic processes such as fatty acid oxidation and glucose regulation. Adiponectin is mainly secreted from the adipose tissues into the bloodstream and it is found to be abundant in the plasma when compared to many other hormones. Acrp30 is a protein hormone which regulates metabolic processes such as fatty acid oxidation and glucose regulation. It has a molecular weight of 69,874 Da.

Adiponectin mainly found to be abundant in the plasma when compared to many other hormones. The levels are found high in the morning before breakfast, after fasting for 12 hours, after an overnight fast; this indicates that it affects the general metabolic rate of an individual by altering the number of calories used throughout the day. With the pancreas being stimulated by acrp30, it produces glucose out of its stored glycogen. This is then used as fuel for cells in the body, mainly muscles and nerves. Adiponectin also affect brain functions such as sleep and feeding behaviours. The hormone affects all three phases of the thyroid- anabolism, catabolism, homeostasis; regulating these phases can alter energy levels in different parts of the body including normalizing metabolic rates and maintaining basal metabolic rates which are generally low during fasting but high when food has been eaten to compensate for the low metabolic rate.

The use of rats and mice offer a suitable model organisms in order to carry out basic research and many pre-clinical studies, this is in addition to the number of different cell culture models and studies using human patients. Transgenic mice which display increased levels of adiponectin are also found to show impaired adipocyte differentiation and there also increased levels of energy expenditure that is linked with protein uncoupling. The use of rodent models can also be useful as they may have similar physiological and molecular characteristics to humans, however studies using human models may be more appropriate for exploring differences in pathology.

INTENDED USE

Mouse adiponectin ELISA kit is a procedure intended for measuring quantitative concentrations of Acrp30 (adiponectin, AdipoQ) in mouse serum and plasma samples. This assay has a minimum sensitivity detection limit of < 0.008 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Plate: coated with anti-adiponectin antibody.
Standards A-F: Lyophilised. Standard values are 0.025, 0.075, 0.15, 0.3, 0.65 and 1 ng/ml.
Dilution Buffer.
Control Serum KS1 & KS2.
Antibody-POD-Conjugate AK.
Washing Buffer (20x Concentrated).
Substrate.
Stop Solution.
Sealing Tape: For covering of the microtiter plate (2x adhesive).

SENSITIVITY

The minimum sensitivity detection limit of adiponectin (mouse) using current mouse adiponectin ELISA kit was approximately 0.008 ng/ml. The dynamic range for this assay is 0.025 – 1.0 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.008 ng/mL.
– Inter Assay Variability: 1.12 – 8.04 µg/mL (mean).
– Intra Assay Variability: 1.712 – 7.395 µg/mL (mean).

REFERENCES

Accumulation of adiponectin in inflamed adipose tissues of obese mice. Metabolism. (2014) 63 (4): 542-53. Nakatsuji H., et al.
Role of adiponectin in adipose tissue wound healing. Genet Mol Res. (2015) 14 (3): 8883-91. Jin C.E., et al.
Protease-based ELISA for selective quantification of mouse high-molecular-weight-adiponectin. Clin Chim Acta. (2009) 401 (1-2): 181-3. Ebinuma H. and Matsuo M.
Adiponectin is partially associated with exosomes in mouse serum. Biochem Biophys Res Commun. (2014) 448 (3): 261-6. Phoonsawat W., et al.

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