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Rat/Mouse Amyloid Beta (1-42) ELISA Kit (Aβ 1-42)

Full Name: Rat/Mouse Amyloid Beta (1-42) ELISA Kit (Aβ 1-42)
Reactivity: Rat, Mouse
Sample Type: Plasma, Serum
Sensitivity: 0.05 pg/ml

BACKGROUND

Senile plaques that have been identified consist of both amyloid beta (Aβ40 and Aβ42), whereas the vascular amyloid predominately contains the shorter Aβ40. The exact composition of these plaque types has been debated, but one prominent theory is that senile plaques are not as toxic as other plaque types. Aβ42 helps regulate the function of cells called neurons by binding to a receptor called ApoE4. This binding allows for a signalling pathway to be activated within the cell. There are numerous different plaque forms besides A42 and ApoE4 that can result in Alzheimer’s disease. Senile plaques, neurofibrillary tangles, and neuritic plaques are examples of these. The last type is generally found in areas where brain lesions have occurred and it is thought to be caused by amyloid beta 42 as well as tau protein.

Also, due to its more hydrophobic nature amyloid beta (1-42) is regarded as the more amyloidogenic form of the peptide. Functions of this include; kinase enzyme activation, cholesterol transport regulation, transcription factor function, oxidative stress protection and anti-microbial activity. Crystallization of amyloid beta (1-42) was achieved by adding glycine and the crystal structure was solved by x-ray crystallography. The overall structure of amyloid beta is a cubic or octahedral shape with a hydrophobic core and a hydrophilic surface region. The hydrophobic core is composed of two rings, each consisting of two disulfide bridges, which are also involved in making up the core pentapeptide ring structure and hold together all four subunits in the bioactive conformation. Amyloid beta is composed of 4 types of protein subunits. The core pentapeptide ring consists of 43 amino acids and binds the four subunits of amyloid beta together in an octahedral structure.

INTENDED USE

Rat/mouse amyloid beta (1-42) ELISA kit can be used for detecting quantitative concentrations of Aβ 1-42 (amyloid beta, Abeta, beta-amyloid) in rat and mouse EDTA plasma or serum samples. This assay has a minimum sensitivity detection limit of 0.05 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with Anti-amyloid beta (38-42).
  • Enzyme Conjugate Concentrate (30x Concentrated).
  • Standard.
  • Assay Buffer.
  • Conjugate Buffer.
  • TMB Substrate Solution.
  • Stop Solution.
    Wash Buffer (40x).

SENSITIVITY

The minimum sensitivity detection limit of beta-amyloid (Aβ, Abeta) using current rat/mouse amyloid beta (1-42) ELISA kit was approximately 0.05 pg/ml. The dynamic range for this assay is 1.56 – 100.0 pg/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.05 pg/mL
– Specificity: Mouse/Rat Aβ (1-42)(100%), Human Aβ (1-42)(1.3 %) and Mouse/Rat Aβ (1-40)(<0.1 % ).
– Intra Assay: 3.4 – 5.8%
– Inter Assay: 2.8 – 8.0%

REFERENCES

  1. Technical performance of a novel, fully automated electrochemiluminescence immunoassay for the quantitation of β-amyloid (1-42) in human cerebrospinal fluid. Alzheimers Dement. (2016) 12 (5): 517-26. Bittner T., et al.
  2. Biomarkers in the diagnosis and management of Alzheimer’s disease. Metabolism. (2015) 64 (3 Suppl 1): S47-50. Review. Wurtman R.
  3. The membrane-active amphibian peptide caerin 1.8 inhibits fibril formation of amyloid β1-42. Peptides. (2015) 73: 1-6. Liu Y., et al.
  4. Inhalation of coriander volatile oil increased anxiolytic-antidepressant-like behaviors and decreased oxidative status in beta-amyloid (1-42) rat model of Alzheimer’s disease. Physiol Behav. (2014) 131: 68-74. Cioanca O., et al.
  5. Cellular Model of Alzheimer’s Disease: Aβ1-42 Peptide Induces Amyloid Deposition and a Decrease in Topo Isomerase IIβ and Nurr1 Expression. Curr Alzheimer Res. (2017) 14 (6): 636-644. Terzioglu-Usak S., et al.

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