TGF-Beta ELISA Kit (Transforming Growth Factor Beta)

INTENDED USE

Human TGF-beta ELISA kit can be used to detect concentrations of transforming growth factor beta (TGF-beta, TGF-β1, CED, DPD1) present in human serum, biological fluids, tissue homogenates and plasma samples. This assay has a minimum sensitivity detection limit of 18.75 pg/ml.

INTRODUCTION

Transforming growth factor beta (TGF-beta) is involved in the regulation of cell proliferation and differentiation, it also plays an important role in wound healing and tissue repair processes. It has been shown to be a possible therapeutic target in the treatment of metabolic syndrome, Type 2 diabetes mellitus and vascular complications. The protein is initially synthesized as a precursor molecule that is called pro-TGF-beta, it is then cleaved into the active forms of TGF-beta1, TGF-beta2 and TGF-beta3. It is mainly produced within the intestines, whilst both TGF-beta2 and TGF-beta3 are produced somewhere elsewhere.

The effects of TGF-beta1 mainly occurs in the gut, liver, lungs and kidneys. Whist those of TGF-beta2 and TGF-beta3 it only occurs in the liver and kidneys. It is secreted by a variety of cells, including fibroblasts, keratinocytes and inducible T cells. In addition to its mitogenic effects on progenitor cells and other mesenchymal tissues, it is also an important factor in the development of bone tissue (osteoblasts). It plays a key role in senescence-associated osteoclasts resorption of bone matrix by stimulating p38 MAP kinase signalling pathway which leads to the release of calcium from the mineral compartment, a necessary step for bone resorption.

In the eye, TGF-beta it is secreted by photoreceptors and choroid to maintain the basement membrane and glaucoma filaments, respectively. In the central nervous system, it prevents granule cell death during olfactory bulb development in mice. Alternatively, it has been shown to preserve axotomized hippocampal neurons in culture via CCN3 gene expression.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with TGF-beta
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.
• Pre-process Agent A
• Pre-process Agent B

TYPICAL RESULTS

For this TGF-beta ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TGF-beta, no cross reactivity or interference between TGF-beta and analogues was detected.
– Recovery: Serum (85 – 103%), EDTA Plasma (88 – 104%), Heparin Plasma (86 – 103%).
– Linearity: Serum (87 – 98%), EDTA Plasma (84 – 100%), Heparin Plasma (82 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. TGF- β: an important mediator of allergic disease and a molecule with dual activity in cancer development. J Immunol Res. (2014) 2014: 318481. Tirado-Rodriguez B., et al.
2. Transforming growth factor-beta (TGF-β) isoforms in wound healing and fibrosis. Wound Repair Regen. (2016) 24 (2): 215-22. Lichtman M.K., et al.
3. TGF-beta and immune cells: an important regulatory axis in the tumor microenvironment and progression. Trends Immunol. (2010) 31 (6): 220-7. Yang L., et al.
4. Engineering TGF-β superfamily ligands for clinical applications. Trends Pharmacol Sci. (2014) 35 (12): 648-57. Kwiatkowski W., et al.

ADDITIONAL INFORMATION

• Full Name: TGF-Beta ELISA Kit (Transforming Growth Factor Beta)
• Reactivity: Human
• Sample Type: Serum, Plasma, Tissue Homogenates, Biological Fluids
• Sensitivity: 18.75 pg/ml

OTHER RELATED ELISA KITS

• TGF-Beta2 ELISA Kit
• Mouse IL-23 ELISA Kit
• Mouse MCP-1 ELISA Kit

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