IL-12 ELISA Kit (Interleukin 12)

INTENDED USE

Human IL-12 ELISA kit can be used for detecting interleukin 12 (IL-12, IL-12p70, IL-12p35) present in human samples of serum, tissue homogenates, biological fluids, and plasma.

INTRODUCTION

Interleukin 12 can bind to specific receptors that are mostly found in T cells and NK cells. The signalling cascade that is induced by binding of the receptors to their respective ligand involves a G protein activating protein called Gq, which stimulates phospholipase C (PLC), leading to release of intracellular calcium. This results in cytosolic calcium levels rising leading to changes in the membrane potential of cells that ultimately leads to cell activation. IL-12 is a cytokine (a signalling protein that regulates the way cells function) that is produced by T helper cells and natural killer cells. It is essential for the maturation of naive and memory T lymphocytes, which are crucial to a healthy immune response. It plays a role in stimulating activation of macrophages and migration of neutrophils to sites with bacterial infection.

IL-12 main function is in the defence against intracellular pathogens, especially mycobacterium species. It is widely regarded as a vital regulator of Th1, activated T cell and NK cell survival. Other roles include modulation of CTLs and NK cells cytotoxic activity. There is evidence to indicate that IL-12 can act as an adjuvant during hormonal immunity through the enhancement of IgG2a and IgG2b antibody formation via B cells. The TLR-12 main function is to detect cell-damaging agents, especially microbe associated molecular patterns (MAMPs). It is an important sensor of bacterial infection in the absence of any other pathway for monitoring.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti IL-12 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this interleukin 12 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125.0, 250.0, 500.0, 1000.0 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-12, no cross reactivity or interference between IL-12 and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (87 – 103%), Heparin Plasma (86 – 102%).
– Linearity: Serum (88 – 104%), EDTA Plasma (82 – 101%), Heparin Plasma (83 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Antitumor effects of interleukin-12 (IL-12): applications for the immunotherapy and gene therapy of cancer. Gene Ther. (1995) 2 (2): 96-106. Tahara H. and Lotze M.T.
2. IL-12 family cytokines: immunological playmakers. Nat Immunol. (2012) 13 (8): 722-8. Vignali D.A., et al.
3. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat Rev Immunol. (2003) 3 (2): 133-46. Trinchieri G.
4. New insights into IL-12-mediated tumor suppression. Cell Death Differ. (2015) 22 (2): 237-46. Tugues S., et al.

ADDITIONAL INFORMATION

• Full Name: IL-12 ELISA Kit (Interleukin 12)
• Reactivity: Human
• Sample Type: Serum, Plasma, Tissue Homogenates, Biological Fluids
• Sensitivity: 9.375 pg/ml

OTHER RELATED ELISA KITS

• IL-10 ELISA Kit
• IL-1 Beta ELISA Kit
• sICAM-1 ELISA Kit