Giardia Lamblia ELISA Kit

INTENDED USE

Human Giardia lamblia ELISA kit is intended for analysing in-vitro levels of giardia lamblia specific antigens using faecal (stool) samples. This assay has a minimum analytical sensitivity limit of 0.08 OD units.

BACKGROUND

Giardiasis is an infection of the small intestine, this can be caused by tiny microscopic parasite that are known as Giardia lamblia. It is a flagellated parasite that is able to reproduce by binary fission through attaching to the epithelium using a ventral adhesive disc. This is confined to the lumen of the small intestines and is not able to spread via the blood steam to other parts of the body or gastrointestinal tract. It can exists in two forms, either as the active form which is called a trophozoite or the inactive form that is known as a cyst.

Diagnosis of giardiasis is carried out using a number of non-invasive and invasive methods, one of the most common techniques involves using microscopic examination of stools samples. The development of ELISA procedures have provided an alternative method with is comparable sensitivity to microscopic examinations. These are fairly simple to perform and also do not require the need to observe intact organisms.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Giardia Lamblia Coated Microtiter Strips.
• Control 1 (Negative).
• Control 2 (Positive).
• Wash Buffer (20x).
• Anti-Giardia Enzyme Conjugate.
• Specimen Diluent.
• Chromogen/Substrate.
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human giardia lamblia specific antigens using current Giardia lamblia ELISA kit was 0.08 OD units. The dynamic range for this ELISA assay is to a specific cut-off point.

REFERENCES

1. An integrated microfludic device for culturing and screening of Giardia lamblia. Exp Parasitol. (2014) 137: 1-7. Zheng G.X., et al.
2. Giardia mitosomal protein import machinery differentially recognizes mitochondrial targeting signals. Infect Disord Drug Targets. (2014) 14 (1): 23-9. Nyindodo-Ogari L., et al.
3. Modeling and analysis of propulsion in the multi-flagellated mico-organism Giardia-lamblia. Phys Rev E Stat Nonlin Soft Matter Phys. (2013) 88 (1): 012726. Lenaghan S.C., et al.
4. A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol. (2016) 194 (1): 38-48. Brown J.R., et al.
5. A comparison of the Giardia lamblia-trophozoite and cyst transcriptome using microarrays. BMC Microbiol. (2011) 11: 91. Faghiri Z. and Widmer G.
6. Biology of Giardia lamblia. Clin Microbiol Rev. (2001) 14 (3): 447-75. Review. Adam R.D.

ADDITIONAL INFORMATION

• Full Name: Giardia Lamblia ELISA Kit
• Reactivity: Human
• Sample Type: Stool
• Sensitivity: 0.08 OD units

OTHER RELATED ELISA KITS

• Echinococcus IgG ELISA Kit
• Entamoeba IgG ELISA Kit
• Ascaris Lumbricoides IgG ELISA Kit