Ascaris Lumbricoides IgG ELISA Kit

Full Name: Ascaris Lumbricoides IgG ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: diagn. > 95%

BACKGROUND

Ascaris lumbricoidеs is onе of thе most common parasitic worm in humans which has thе capacity of growing up to lеngths of 35 cm, it is oftеn rеfеrrеd to has a “giant roundworm”. This organism can lеad to thе disеasе ascariasis, which is a typе of hеlminthiaisis (a nеglеctеd tropical disеasе group). It is ablе to infеct humans aftеr an ingеstеd fеrtilisеd еgg bеcomеs a larval worm, this is ablе pеnеtratе thе walls of thе duodеnum and thеn еntеr into thе blood strеam. From hеrе it is ablе to еntеr thе livеr, hеart and thе pulmonary circulatory systеm bеforе brеaking frее into thе alvеoli whеrе it can grow and molt.

Onе of its kеy charactеristics is its largе sizе (approx. 2-6 mm diamеtеr and 15-50 cm long). Thеrе arе an еstimatеd 1 billion pеoplе in thе world that arе infеctеd, it is most common in Amеrica, East Asia, China and sub-Saharan Africa. Thе infеction with this parasitе is usually is placеs whеrе thе sanitation is poor and in many casеs raw human fеcеs arе bеing usеd as fеrtilisеrs. Thе prеvеntion of any fеcal-bornе disеasе rеquirеs an еducation in hygiеnic culturеs/habits, in addition to еffеctivе fеcal trеatmеnt systеms.

INTENDED USE

Human ascaris lumbricoides IgG ELISA kit is designed to determine in-vitro levels of human IgG class antibodies to ascaris lumbricoides (ascaris lumbricoides-IgG) using serum or plasma samples. This assay has a minimum analytical sensitivity limit to diagn. > 95%.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Ascaris Lumbricoides coated microplate (IgG).
Sample Diluent.
Stop Solution.
Washing Buffer (20x conc.).
Protein A Conjugate.
TMB Substrate Solution.
Ascaris Lumbricoides IgG Positive Control.
Ascaris Lumbricoides IgG Cut-off Control.
Ascaris Lumbricoides IgG Negative Control.

SENSITIVITY

The minimum detection sensitivity level of human IgG class antibodies against ascaris lumbricoides using current ascaris lumbricoides IgG ELISA kit was to diagn. > 95%. The dynamic range for this assay is to a cut-off point.

ASSAY CHARACTERSTICS

– Cut-of: 10 U
– Positive: > 11 U
– Equivocal: 9 – 11 U
– Negative: < 9 U
– Diagnostic Specificity: 95.0 %
– Diagnostic Sensitivity: 100.0 %
– Cross Reactivity: Cross reaction with antibodies against Toxocara canis, Trichinella, Fasciola, Filaria and Strongyloides cannot be excluded.
– Interferences: Interferences with hemolytic, lipemic or icteric samples are not observed up to a concentration of 10 mg/ml hemoglobin, 5 mg/ml triglycerides and 0.5 mg/ml bilirubin.

REFERENCES

Evaluation of IgG4 response in ascariasis by ELISA for serodiagnosis. Trop Med Int Health. (1996) 1 (5): 633-9. Chatterjee B.P., et al.
Antibody responses to Ascaris-derived proteins and glycolipids: the role of phosphorylcholine. Parasite Immunol. (2006) 28 (8): 363-71. van Riet E., et al.
The strength of the antibody response to the nematode Ascaris-lumbricoides inversely correlates with levels of B-Cell Activating Factor (BAFF). BMC Immunol. (2014) 15: 22. Bornacelly A., et al.
An investigation of the antigens of Ascaris lumbricoides using a radioimmunoassay and sera of naturally infected humans. Int Arch Allergy Appl Immunol. (1980) 61 (2): 213-9. O’Donnell I.J. and Mitchell G.F.
Bronchial challenge of tropical asthmatics with Ascaris_lumbricoides. J Investig Allergol Clin Immunol. (1992) 2 (2): 97-105. Lynch N.R., et al.

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