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Prolactin (PRL) ELISA Kit (Canine)

  • Created on the 29 August, 2018.

INTENDED USE

Canine prolactin ELISA kit is a procedure for measuring quantitative amounts of prolactin (PRL) using canine (dog) serum samples. This assay has a minimum sensitivity detection limit of 0.4 ng/ml.

BACKGROUND

Canine prolactin (cPRL) is a single polypeptide chain which is approx. 22,000 in molecular mass, there are significant differences in the amino acid sequence of prolactin which is displayed in different species. For example; human and canine prolactin differ in about 60% of all the residues. One of the most important functions of prolactin is to stimulate lactation and mammary gland growth. This is evident by the fact that during pregnancy, the levels of prolactin (PRL) in canine blood is increased slightly whereas during lactation, there is a significant increase. There are many other physiological actions of prolactin such as: affecting electrolyte and water balance, vital for stress hormones, metabolism and gonadal function. The measurement of prolactin (PRL) has also be critical during the therapeutic control of hyper-prolactinemia.

SENSITIVITY

The minimum sensitivity detection limit of dog prolactin (PRL) using current canine prolactin ELISA kit is 0.4 ng/ml. The dynamic range for this assay is 2.5 – 80.0 ng/ml.

REFERENCES

  1. Heterogeneity of circulating prolactin in the bitch. Reprod Nutr Dev. (2001) 41 (6): 505-11. Gobello C., et al.
  2. Clinical and prognostic implications of serum and tissue prolactin levels in canine mammary tumours. Vet Rec. (2014) 175 (16): 403. Queiroga F.L., et al.
  3. Determination of canine prolactin by use of a commercial heterologous enzymeimmunoassay kit. Zentralbl Veterinarmed A. (1996) 43 (2): 65-74. Høier R. and Jensen A.L.
  4. Molecular cloning and expression of canine prolactin gene. Exp Anim. (2010) 59 (5): 643-6. Hashimoto O., et al.
  5. Role of steroid hormones and prolactin in canine mammary cancer. J Steroid Biochem Mol Biol. (2005) 94 (1-3): 181-7. Queiroga F.L., et al.

ADDITIONAL INFORMATION

  • Full Name: Prolactin (PRL) ELISA Kit (Canine)
  • Reactivity: Canine
  • Sample Type: Serum
  • Sensitivity: 0.4 ng/ml

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