Rat Prolactin ELISA Kit (PRL)

Full Name: Rat Prolactin ELISA Kit (PRL)
Reactivity: Rat
Sample Type: Serum
Sensitivity: 0.6 ng/ml

BACKGROUND

Rat prolactin (rPRL) is a hormone of the anterior pituitary that is a single chained polypeptide having a molecular mass of 23,000. It has two subunits or two chains that are linked by disulfide bonds. These chains are linked together by cysteine residues, creating a non-covalently formed dimer known as the active form. This is asymmetrical dimer and contains one helical molecule and one anti-helical molecule. In the anti-helical molecule, there is a sulfhydryl group in position 170. The pituitary gland in the anterior lobe of the human brain secretes rPRL, which is made up of two chains joined by disulfide bonds, into the bloodstream. Protein X binds to one prolactin chain, while protein Y to a different prolactin chain. Then, at one of their cysteine sites, proteins X and Y form a covalent link. This forms an asymmetrical dimer known as the active form which contains one helical molecule and one anti-helical molecule.

Prolactin differs considerably between different species, for example; rat and human prolactin differ by approx. 50% of all residues. Since is highly conserved between different species, it was difficult to elucidate why there are such differences. It has been postulated that there are differences in sensitivity to hormones and receptor occupancy. The protein is encoded by the “PRL” gene located on chromosome 15.

INTENDED USE

Rat prolactin ELISA kit can be used to detect quantitative levels of prolactin (PRL) using rat serum samples. This assay has a minimum sensitivity detection limit of 0.6 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with a monoclonal anti rat prolactin antibody.
• Master Calibrator.
• Enzyme Conjugate.
• Calibrator/Sample Diluent.
• Sample Buffer.
• Substrate (TMB).
• Stop Solution.
• Wash Solution (10x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of rat prolactin (PRL) using current rat prolactin ELISA kit is 0.6 ng/ml. The dynamic range for this assay is 5.0 – 80.0 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.6 ng/ml
– Specificity: Rat Prolactin (100%)
– Intra-Assay: 3.0 – 5.5%
– Inter-Assay: 3.5 – 4.4%
– Recovery: 91.0 – 105.0%
– Linearity: 96.0 – 115.0%
– A high dose hook effect could not be observed.

REFERENCES

1. Regulation of prolactin transformation in the rat pituitary. Ciba Found Symp. (1992) 168: 69-80. Review. Grosvenor C.E. and Mena F.
2. Extraction of rat pituitary prolactin for radioimmunoassay. J Endocrinol Invest. (1990) 13 (3): 205-8. Torres A.I., et al.
3. Studies on the development of growth hormone and prolactin_cells in the rat pituitary gland by in situ hybridization. Cell Tissue Res. (1989) 255 (1): 23-8. Nogami H., et al.
4. Heterogeneity of immunoreactive prolactin in the rat brain. Biochem Biophys Res Commun. (1988) 150 (2): 599-604. DeVito W.J.
5. Biological, immunological and biochemical characterization of cleaved prolactin generated by lactating mammary gland. Biochim Biophys Acta. (1987) 931 (2): 196-204. Vick R.S., et al.
6. Size and charge heterogeneity of pituitary and plasma-prolactin in the male rat. Neuroendocrinology. (1996) 63 (5): 437-45. Briski K.P., et al.

OTHER RELATED ELISA KITS

• Prolactin ELISA Kit (Canine)
• PMSG ELISA Kit (Equine)
• Estradiol ELISA Kit (Rat)