Prolactin (PRL) ELISA Kit (Rat)
INTENDED USE
Rat prolactin ELISA kit can be used to detect quantitative levels of prolactin (PRL) using rat serum samples. This assay has a minimum sensitivity detection limit of 0.6 ng/ml.
BACKGROUND
Rat prolactin (rPRL) is a hormone of the rat anterior pituitary that is a single chained polypeptide having a molecular mass of 23,000. It has been found that prolactin differs considerably between different species, for example; rat and human prolactin differ by approx. 50% of all residues. There is also evidence that the lobe of the rat posterior pituitary contains a prolactin releasing hormone. An important role of prolactin is in the stimulation of mammary gland growth and lactation. High levels of prolactin (PRL) are displayed during lactation, during pregnancy, prolactin levels in the blood can increase, however this elevation can differ greatly between rats. In rats, similar to humans, prolactin is found to exhibit a sleep related diurnal variations. Where nidar value are found in the morning and peak values are displayed in the late afternoon. Finally, in rats, prolactin has a luteotrophic effect and this is not displayed in many of the other species.
RAT PROLACTIN ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Coated with a monoclonal anti-rat prolactin antibody.
- Master Calibrator.
- Enzyme Conjugate.
- Calibrator/Sample Diluent.
- Sample Buffer.
- Substrate (TMB).
- Stop Solution.
- Wash Solution (10x Conc.).
SENSITIVITY
The minimum sensitivity detection limit of rat prolactin (PRL) using current rat prolactin ELISA kit is 0.6 ng/ml. The dynamic range for this assay is 5.0 – 80.0 ng/ml.
ASSAY CHARACTERISTICS
– Rat Prolactin (PRL): ELISA
– Analytical Sensitivity: 0.6 ng/ml
– Specificity: Rat Prolactin (100%)
– Intra-Assay: 3.0 – 5.5%
– Inter-Assay: 3.5 – 4.4%
– Recovery: 91.0 – 105.0%
– Linearity: 96.0 – 115.0%
– A high dose hook effect could not be observed.
REFERENCES
- Regulation of prolactin transformation in the rat pituitary. Ciba Found Symp. (1992) 168: 69-80. Review. Grosvenor C.E. and Mena F.
- Extraction of rat pituitary prolactin for radioimmunoassay. J Endocrinol Invest. (1990) 13 (3): 205-8. Torres A.I., et al.
- Studies on the development of growth hormone and prolactin cells in the rat pituitary gland by in situ hybridization. Cell Tissue Res. (1989) 255 (1): 23-8. Nogami H., et al.
- Heterogeneity of immunoreactive prolactin in the rat brain. Biochem Biophys Res Commun. (1988) 150 (2): 599-604. DeVito W.J.
- Biological, immunological and biochemical characterization of cleaved prolactin generated by lactating mammary gland. Biochim Biophys Acta. (1987) 931 (2): 196-204. Vick R.S., et al.
- Size and charge heterogeneity of pituitary and plasma prolactin in the male rat. Neuroendocrinology. (1996) 63 (5): 437-45. Briski K.P., et al.
ADDITIONAL INFORMATION
- Full Name: Prolactin (PRL) ELISA Kit (Rat)
- Reactivity: Rat
- Sample Type: Serum
- Sensitivity: 0.6 ng/ml
