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Corticosterone ELISA Kit (Rat/Mouse)

INTENDED USE

Rat/mouse corticosterone ELISA kit is intended for detecting quantitative amounts of corticosterone in rat or mouse in samples of serum and plasma. This assay has a minimum sensitivity detection limit of 6.1 ng/ml.

BACKGROUND

Corticosterone (also called 11β,21-dihydroxyprogesterone or 17-deoxycortisol) is produced in the cortex by the adrenal glands, a 21-carbon steroid hormone of the corticosteroid type. It is secreted by the adrenal cortex which is mainly controlled by the ACTH (a pituitary hormone) through a negative feedback mechanism. In rats, it represents one of the most abundant steroid that is circulating, due to the fact that rodents are unable to synthesize cortisol (a major glucocorticoid in human), this is because of a lack of the enzyme C17-Hydroxylase. The levels of corticosterone in nocturnal animals (such as in rats) display a distinct circadian variation with peak values in the latter portion of the day, that is followed by a nadir in the morning and this is thought to play an vital role in sleep-wake cyclus. Corticosterone is important for a large number of activities in rodents. It is vital in regulating protein, carbohydrate and fat metabolism. It is also responsible for influencing the hemopoietic system and can lead to reducing the total number of eosinophils and lymphocytes, but this is to a lesser extent when compared to cortisol. The measurement of corticosterone concentrations in rats is of particular interest to facilities that are conducting neurophysiological research and drug research departments.

RAT/MOUSE CORTICOSTERONE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterplate Strips: Wells coated with polyclonal rabbit anti-corticosterone antibody.
  • Calibrator 0.
  • Calibrator ( 1-5): Concentrations: 15 – 50 – 185 – 640 – 2250 ng/ml
  • Incubation Buffer.
  • Enzyme Conjugate.
  • Corticosterone Conjugated To Horseradish Peroxidise.
  • Substrate Solution (TMB).
  • Stop Solution.
  • Wash Solution (10x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of rat and mouse corticosterone using current rat/mouse corticosterone ELISA kit was approximately 6.1 ng/ml. The dynamic range for this assay is 15.0 – 2,250.0 ng/ml.

ASSAY CHARACTERISTICS

– Rat, Mouse Corticosterone: ELISA
– Analytical Sensitivity: 4.1 ng/ml
– Intra Assay: 2.5 – 8.3%
– Inter Assay: 4.8 – 12.7%
– Recovery: 83 – 117%.
– Linearity: 300 – 382 ng/ml
– Drug Interferences: Until now there are no substance (drug) which are known to influence with the measurement.

REFERENCES

  1. Development of an antigen heterologous enzyme linked immunosorbent assay for measurement of corticosterone in rat serum. J Immunoassay Immunochem. (2011) 32 (3): 244-57. Shrivastav T.G., et al.
  2. Effects of different timing of stress on corticosterone, BDNF and memory in male rats. Physiol Behav. (2015) 139: 459-67. Radahmadi M., et al.
  3. High plasma corticosterone levels persist during frequent automatic blood sampling in rats. In Vivo. (2005) 19 (5): 815-9. Abelson K.S., et al.
  4. Development of an enzyme-linked immunosorbent assay for serum 11-dehydrocorticosterone in rat and mouse. Biol Pharm Bull. (2007) 30 (3): 403-9. Tagawa N., et al.

ADDITIONAL INFORMATION

  • Full Name: Corticosterone ELISA Kit (Rat/Mouse)
  • Reactivity: Rat, Mouse
  • Sample Type: Plasma, Serum
  • Sensitivity: 6.1 ng/ml

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