Progesterone ELISA Kit (Rat/Mouse)
INTENDED USE
Rat/mouse progesterone ELISA kit is designed to quantitatively detect concentrations of mouse or rat progesterone using plasma or serum samples. This assay has a minimum sensitivity detection limit of 0.04 ng/ml.
BACKGROUND
Progesterone which is also known as 4-pregnene-3, 20-dione is a C21 steroid hormone which consists of a double bond between C-4 and C-5, it also contains a keto group at the C-3 position. It is a female sex hormone which play a critical role during ovulation, fertility and menopause. One of progesterone main function is to prepare the endometrium for implanting the blastocytes and for maintaining pregnancy. In vivo studies have indicated that it can act as an anti-glucocorticoid in the adipose tissues of rats, also it has been found to affect adipose metabolism if this process is attenuated. Progesterone alone has been demonstrated to be a vital agent for managing postmenopausal osteoporosis. Finally, in female rodents, measurement of progesterone is regarded as a crucial maker to evaluate and monitor the reproductive functions and pregnancy.
RAT/MOUSE PROGESTERONE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Wells: Coated with polyclonal anti-progesterone antibody.
- Calibrator 0.
- Calibrator (Calibrator 1-5): Concentrations: 0.4, 1.5, 6.5, 25, 100 ng/ml.
- Enzyme Conjugate.
- TMB Substrate Solution.
- Stop Solution.
- Wash Solution (10x Conc.).
SENSITIVITY
The minimum sensitivity detection limit of mouse or rat progesterone using current rat/mouse progesterone ELISA kit is 0.04 ng/ml. The dynamic range for this assay is 0.4 – 100.0 ng/ml.
ASSAY CHARACTERISTICS
– Mouse, Rat Progesterone: ELISA
– Analytical Sensitivity: 0.156ng/ml
– Intra Assay: 5.0 – 11.5%
– Inter Assay: 6.1 – 13.6%
– Recovery: 78 – 108%
– Linearity: 92 – 112%
REFERENCES
- Progesterone attenuates hemorrhagic transformation after delayed tPA treatment in an experimental model of stroke in rats: involvement of the VEGF-MMP pathway. J Cereb Blood Flow Metab. (2014) 34 (1): 72-80. Won S., et al.
- Progesterone binding in the mouse and rat uterus. Endocrinology. (1972) 91 (3): 738-46. Feil P.D., et al.
- Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury. Neuroscience. (2013) 231: 111-24. Meffre D., et al.
- Rethinking progesterone regulation of female reproductive cyclicity. Proc Natl Acad Sci U S A. (2016) 113 (15): 4212-7. Kubota K., et al.
- Metabolism of progesterone in mouse brain. J Steroid Biochem Mol Biol. (1994) 50 (3-4): 213-7. Carey M.P., et al.
ADDITIONAL INFORMATION
- Full Name: Progesterone ELISA Kit (Rat/Mouse)
- Reactivity: Rat, Mouse
- Sample Type: Serum, Plasma
- Sensitivity: 0.04 ng/ml
