Rat/Mouse Testosterone ELISA Kit

Full Name: Rat/Mouse Testosterone ELISA Kit
Reactivity: Mouse, Rat
Sample Type: Serum, Plasma
Sensitivity: 0.066 ng/ml

BACKGROUND

Testosterone (17beta-hydroxy-4-androstene-3-one) belongs to the androgen family, which is composed of a C19 steroid containing keto and hydroxyl groups and it is one of the most effective natural hormones. The biosynthesis of testosterone has been found to coincide with the spermatogenesis and fetal Leydig cell differentiation in the male rat, also the capacity of Leydig cells to produce and secrete testosterone is far higher in young when compared to older rats. The principal negative feedback loop regulating the release of testosterone is a hypothalamic-pituitary-testis axis. These mechanisms are termed as the innate control of testicular function by sex steroids.

Testosterone is likewise recognised to be secreted in the course of overdue gestational and neonatal durations which could motive good sized stage of mind sexual dimorphism in rats. It can exert a number of varying influencing over sexual behaviour, cardiovascular health, overall energy, bone integrity, muscle strength and mass. The primary role is in developing secondary male sex characteristics and it can be also be useful in evaluating the hypogonadal states. The girl reproductive device often relies upon on testosterone, which is likewise generated via way of means of women. The levels can vary based on the phase of their menstrual cycle and sex-atypical symptoms such as diminished libido, infertility, irregular menstruation and menopause can lead to medical evaluation.

INTENDED USE

Rat/mouse testosterone ELISA kit is a competitive immunoassay which is designed for detecting quantitative levels of testosterone in mouse or rat plasma or serum samples. This assay has a minimum sensitivity detection limit of 0.066 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Strips: Coated monoclonal anti-testosterone antibody.
• Calibrator O.
• Calibrator 1-5: Concentrations 0.1, 0.4, 1.5, 6.0, 25.0 ng/ml.
• Incubation Buffer.
• Enzyme Conjugate.
• TMB Substrate Buffer.
• Wash Buffer (10x Concentration).
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of mouse and rat testosterone using current rat or mouse testosterone ELISA kit was approximately 0.066 ng/ml. The dynamic range for this assay is 0.1 – 25.0 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.066ng/ml
– Intra Assay: 6.50 – 11.07%
– Inter Assay: 9.3 – 11.3%
– Recovery: 85 – 110%
– Drug Interferences: No substances are known to influence measurement. However, haemolysed and lipemic samples can lead to false results.

REFERENCES

1.  Differential effects of nonylphenol on testosterone secretion in rat Leydig cells. Toxicology. (2010) 268 (1-2): 1-7. Wu J.J., et al.
2. The effects of chronic administration of testosterone propionate with or without estradiol on the sexual behavior and plasma steroid levels of aged female rats. Endocrinology. (2012) 153 (12): 5928-39. Jones S.L., et al.
3. Enzyme immunoassay of 17 beta-estradiol, estrone conjugates, and testosterone in urinary and fecal samples from male and female mice. Horm Metab Res. (2001) 33 (11): 653-8. Muir C., et al.
4. The anxiolytic effect of testosterone in the rat is mediated via the androgen receptor. Pharmacol Biochem Behav. (2012) 102 (2): 191-5. Hodosy J., et al.

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