Rat TSH ELISA Kit (Thyroid Stimulating Hormone)

Full Name: Rat TSH ELISA Kit (Thyroid Stimulating Hormone)
Reactivity: Rat
Sample Type: Serum
Sensitivity: 0.1 ng/ml

BACKGROUND

TSH (thyrotropin or Thyroid Stimulating Hormone) plays a role in monitoring the standard amount of iodothyronines (T3 and T4) in the bloodstream. Its presence ensures proper functioning of the thyroid gland. Its levels act as a feedback controller of the thyroid, which will eventually cause the release of T3 and T4 when needed. There are two types of this hormone fast-acting and slow-acting. The fast acting kind is released every 3 minutes until it reaches its peak between 30 to 60 minutes after you’re exposed to it, while the slow-acting is released in a slower manner throughout an entire day.

TSH mechanism process for controlling thyroid function in rats is found to be identical to that of humans and many other mammalian species. Due to the identical similarity between human and rat thyroid physiology, this makes the use of rats as an ideal model when evaluating the effects of new drugs on thyrometabolic status. It exerts its physiological effects through the activation of a signalling cascade that increases thyroid hormone synthesis (via increased transcription and translation) and secretion from the thyroid glands. In rats, low levels of circulating TSH lead to increased synthesis and secretion from the thyroid gland and reduced free thyroxine in blood serum. In humans, a similar mechanism of action is also seen with low levels of circulating TSH leading to decreased free thyroxine in blood serum.

INTENDED USE

Rat TSH ELISA kit is intended for analysing quantitative amounts of thyroid stimulating hormone (TSH, thyrotropin) present in rat serum. This assay has a minimum sensitivity detection limit of 0.1 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with a monoclonal anti-rat TSH antibody.
• Master Calibrator (80 ng, Lyophilized): Reconstituted to calibrators A-G with concentrations of 0, 2.5, 5, 10, 20, 40, 80ng/ml.
• Enzyme Conjugate.
• TMB Substrate Solution.
• Stop Solution.
• Wash Solution (10x Concentrated).
• Calibrator/Sample Diluent.
• Adhesive Cover.

SENSITIVITY

The minimum sensitivity detection limit of thyroid stimulating hormone (thyrotropin) using current rat TSH ELISA kit is 0.1 ng/ml. The dynamic range for this assay is 2.5 – 80.0 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.081 ng/ml
– Specificity (Cross Reaction): Rat TSH (100%), Rat LH (1.6 – 2.8%), Rat FSH (0.3%).
– Intra – Assay: 1.8 – 3.4%
– Inter – Assay: 4.6 – 8.8%
– Linearity: 111.0 – 123.0%

REFERENCES

1. Effect of endogenous TSH on the in vitro responsiveness and binding of TSH in rat thyroid tissue. Endocr Res Commun. (1980) 7 (4): 205-19. Holmes S.D. and Field J.B.
2. TSH binding proteins in rat and human serum. Acta Endocrinol (Copenh). (1987) 115 (4): 497-506. Spira O., et al.
3. Discordant effects of thyrotropin (TSH)-releasing hormone on pre- and posttranslational regulation of TSH biosynthesis in rat pituitary. Endocrinology. (1986) 119 (1): 343-8. Lippman S.S., et al.
4. Neurotensin regulation of TSH-secretion in the rat. Biochem Biophys Res Commun. (1983) 113 (1): 248-54. Sheppard M.C., et al.
5. Plasma and pituitary thyrotrophin (TSH) in severe and prolonged hypothyroidism, as studied in the rat. Clin Endocrinol (Oxf). (1977) 6 (1): 49-56. Cacicedo L., et al.
6. Rapid proliferative response of rat thyroid gland to a single injection of TSH in vivo. J Endocrinol. (1989) 121 (1): 27-30. Bybee A. and Tuffery A.R.

OTHER RELATED ELISA KITS

• TSH ELISA Kit
• TSH ELISA Kit (Canine)
• Throglobulin (hTG) ELISA Kit