TSH ELISA Kit (Canine)


Canine TSH ELISA kit can be used for quantitative detection of thyroid stimulating hormone (TSH, thyrotrophin) in samples of canine (dog) plasma and serum. This assay has a minimum sensitivity detection limit of 0.01 ng/ml.


Thyroid stimulating hormone (TSH, thyrotropin) is a glycoprotein which is made by the anterior pituitary gland. The function of dog TSH is identical to what is found in other mammalian species, such as humans. One of the primary functions includes helping to maintain circulating levels of T3 and T4, this process is regulated through a negative feedback mechanism. A common endocrine disorder which can be found in dogs is hypothyroidism, majority of these cases present in canines are considered to be primary in nature and these are caused by impaired production of T3 and T4. In many of cases, hypothyroidism in dogs can be identified through clinical history or by the detection of lowered thyroid hormone levels. One thing to bear in mind, suppressed thyroid levels are considered as non-specific indicators of the disease, due to the fact this can also be observed in non-thyroid conditions.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with an anti-canine monoclonal TSH antibody.
  • Calibrators (0-6): Lyophilized, highly purified in canine serum. The concentrations are 0, 0.2, 0.46, 1.05, 2.2 and 5.2 ng/ml.
  • Enzyme Labelled Antibody.
  • TMB Substrate Solution.
  • Stop Solution.
  • Wash Buffer (10x Concentrated).


The minimum sensitivity detection limit of dog thyroid stimulating hormone, (thyrotrophin) using current canine TSH ELISA kit is 0.01 ng/ml. The dynamic range for this assay is 0.2 – 5.2 ng/ml.


– Canine Thyroid Stimulating Hormone (TSH): ELISA
– Analytical Sensitivity: 0.01 ng/ml
– Specificity: Highly specific for canine TSH.
– Intra – Assay: 3.1 – 6.8%
– Inter – Assay: 5.4 – 8.0%
– Recovery: 95.0 – 101.0%
– Linearity: 93.0 – 113.0%


  1. Prevalence of thyroglobulin autoantibodies detected by enzyme-linked immunosorbent assay of canine serum in hypothyroid, obese and healthy dogs in Japan. Res Vet Sci. (2004) 76 (2): 129-32. Lee J.Y., et al.
  2. Evaluation of canine serum thyrotropin (TSH) concentration: comparison of three analytical procedures. J Vet Diagn Invest. (2001) 13 (2): 106-10. Marca M.C., et al.
  3. Impaired TSH secretion during sustained hyperglucagonemia in anesthetized dogs. Thyroid. (1995) 5 (5): 389-93. Kabadi U. and Fragstedt L 2nd.
  4. Diagnosis of canine hypothyroidism. Perspectives from a testing laboratory. Vet Clin North Am Small Anim Pract. (2001) 31 (5): 951-62, vii. Review. Kemppainen R.J. and Behrend E.N.
  5. Biological variation of canine serum thyrotropin (TSH) concentration. Vet Clin Pathol. (1999) 28 (1): 16-19. Iversen L., et al.


  • Full Name: TSH ELISA Kit (Canine)
  • Reactivity: Canine
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.01 ng/ml



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