Canine TSH ELISA Kit (Thyroid Stimulating Hormone)

Full Name: Canine TSH ELISA Kit (Thyroid Stimulating Hormone)
Reactivity: Canine
Sample Type: Serum, Plasma
Sensitivity: 0.01 ng/ml

BACKGROUND

Thyroid stimulating hormone (TSH or thyrotropin), is a glycoprotein which is produced by the anterior pituitary gland. The main function includes helping to maintain the circulating levels of T3 and T4. This process is regulated through a negative feedback mechanism. A common endocrine disorder which can be found in dogs is hypothyroidism, majority of these cases present in canines are considered to be primary in nature and these are caused by impaired production of T3 and T4. TRH also has antagonistic effects on endogenous thyrotrophin-releasing hormone, which would cause a decreased in T4 levels. The metabolism of carbohydrates, cognition, exercise tolerance, heart rate and body temperature are all regulated by thyroid hormones. T4 has the ability to control how quickly cells divide. Thyroxine is created by converting T4 into the active form. The anterior pituitary gland releases TSH when TRH, a hormone, is released.

Hypothyroidism in dogs can be identified through clinical history or by the detection of lowered thyroid hormone levels. One thing to bear in mind, suppressed thyroid levels are considered as non-specific indicators of the disease, due to the fact this can also be observed in non-thyroid conditions. The condition can be diagnosed in dogs through blood tests, ultrasound scans and endoscopy.

INTENDED USE

Canine TSH ELISA kit can be used for quantitative detection of thyroid stimulating hormone (TSH, thyrotrophin) in samples of canine (dog) plasma and serum. This assay has a minimum sensitivity detection limit of 0.01 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Coated with an anti-canine monoclonal TSH antibody.
• Calibrators (0-6): Lyophilized, highly purified in canine serum. The concentrations are 0, 0.2, 0.46, 1.05, 2.2 and 5.2 ng/ml.
• Enzyme Labelled Antibody.
• TMB Substrate Solution.
• Stop Solution.
• Wash Buffer (10x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of dog thyroid stimulating hormone, (thyrotrophin) using current canine TSH ELISA kit is 0.01 ng/ml. The dynamic range for this assay is 0.2 – 5.2 ng/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 0.01 ng/ml
– Specificity: Highly specific for canine TSH.
– Intra – Assay: 3.1 – 6.8%
– Inter – Assay: 5.4 – 8.0%
– Recovery: 95.0 – 101.0%
– Linearity: 93.0 – 113.0%

REFERENCES

1. Prevalence of thyroglobulin autoantibodies detected by enzyme-linked immunosorbent assay of canine serum in hypothyroid, obese and healthy dogs in Japan. Res Vet Sci. (2004) 76 (2): 129-32. Lee J.Y., et al.
2. Evaluation of canine serum thyrotropin (TSH) concentration: comparison of three analytical procedures. J Vet Diagn Invest. (2001) 13 (2): 106-10. Marca M.C., et al.
3. Impaired TSH secretion during sustained hyperglucagonemia in anesthetized dogs. Thyroid. (1995) 5 (5): 389-93. Kabadi U. and Fragstedt L 2nd.
4. Diagnosis of canine hypothyroidism. Perspectives from a testing laboratory. Vet Clin North Am Small Anim Pract. (2001) 31 (5): 951-62, vii. Review. Kemppainen R.J. and Behrend E.N.
5. Biological variation of canine serum thyrotropin (TSH) concentration. Vet Clin Pathol. (1999) 28 (1): 16-19. Iversen L., et al.

OTHER RELATED ELISA KITS

• TSH ELISA Kit (Rat)
• TSH ELISA Kit
• TG-Ab ELISA Kit