Tryptase Alpha/Beta-1 ELISA Kit (TPSAB1)

Full Name: Tryptase Alpha/Beta-1 ELISA Kit (TPSAB1)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.938 ng/ml

INTRODUCTION

TPSAB1, also known as tryptase alpha/beta 1 is an enzyme called serine protease that is found in mast cells in quantities. It plays a role, in both inflammation and the innate immune systems defense against parasites.

In humans TPSAB1 is the member of the tryptase enzyme family stored within mast cell granules. These enzymes make up 20 30% of the protein content in mast cells. When mast cells are activated and release their contents (a process called degranulation) tryptases are secreted. This activation is triggered by the crosslinking of IgE antibodies bound to high affinity IgE receptors. TPSAB1 specifically functions as a serine protease meaning it breaks down peptide bonds in target proteins. By breaking down these substrates TPSAB1 contributes to vasodilation (widening of blood vessels) contraction of muscles, secretion of mucus and increased vascular permeability – all of which contribute to the signs and symptoms associated with responses.

Besides its involvement in diseases TPSAB1 may also play a role in modulating responses to parasites. As a serine protease enzyme TPSAB1 has the ability to directly break down components, on the surface of parasites or cleave host proteins to activate immune pathways.

TPSAB1 also has the ability to activate cells by interacting with protease activated receptors (PARs). This interaction leads to the production of cytokines that trigger inflammation and recruit cells which in turn help combat parasites. The role of TPSAB1, in both inflammation and parasite immunity suggests that it could be a target for therapeutic interventions. By developing inhibitors for TPSAB1 we may be able to alleviate mast cell mediated reactions. Additionally exploring the use of forms of this enzyme could activate PARs. Stimulate protective immune responses against parasites.

INTENDED USE

Human Tryptase Alpha/Beta-1 ELISA kit can measure concentrations of TPSAB1 (Tryptase Alpha/Beta-1, TPS1, TPSAB1) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti- TPSAB1.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this Tryptase Alpha/Beta-1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 1.562, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml.
Reactivity: Human
Sensitivity: 0.938 ng/ml
Range: 1.563 – 100 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TPSAB1, no cross reactivity or interference between TPSAB1 and analogues was detected.
– Recovery: Serum (95 – 103%), EDTA Plasma (85 – 104%), Heparin Plasma (87 – 102%).
– Linearity: Serum (88 – 102%), EDTA Plasma (82 – 94%), Heparin Plasma (80 – 95%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

Tryptase activates the mitogen-activated protein kinase/activator protein-1 pathway in human peripheral blood eosinophils, causing cytokine production and release. J Immunol. (2002) 169 (5): 2662-9. Temkin V., et al.
Localization of rat tryptase to a subset of the connective tissue type of mast cell. J Histochem Cytochem. (1993) 41 (7): 961-9. Chen Z., et al.
Increased TPSAB1 Copy Number in a Family With Elevated Basal Serum Levels of Tryptase. Front Med (Lausanne). (2021) 8: 577081. Hernández-Hernández L., et al.

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