TRACP-5b ELISA Kit (TartRate-Resistant Acid Phosphatase 5b)

INTENDED USE

Human TRACP-5b ELISA kit can measure concentrations of tartRate-resistant acid phosphatase 5b (TRACP-5b, TRatPase, MGC117378) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a sensitivity detection limit of 0.188 mIU/ml.

INTRODUCTION

TRACP-5b, known as tartrate-resistant acid phosphatase 5b, is an enzyme used as a biomarker to assess bone resorption activity. Secreted by activated osteoclasts during bone remodeling, measuring serum levels helps quantify skeletal dynamics in normal physiology and diseases like osteoporosis.

Bone continually remodels itself through osteoclast-mediated resorption of old tissue and osteoblast formation of new bone. Osteoclast activation secretes TRACP-5b to degrade and solubilize bone mineral to initiate skeletal framework teardown. Once released into circulation, the serum levels are used to quantify the current rate of bone matrix catabolism by skeletal osteoclasts.

Elevated serum TRACP-5b indicates heightened osteoclastic resorption outpacing bone formation. This imbalance is favoring breakdown over regeneration and it reflects poorly controlled skeletal remodeling that weakens bone over time. In particular, postmenopausal women exhibit increased levels in line with excessive resorption from estrogen-deficient bone loss. Monitoring serum levels allows early detection of accelerated bone loss well before fragility fractures occur. The enzyme marker gauges patient response to osteoporosis therapies as well. Anti-resorptive treatments like bisphosphonates are used to reduce TRACP 5b levels along with slowing skeletal deterioration, affirming osteoclast inhibition.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-TRACP-5b.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this TRACP-5b ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 mIU/ml.
Reactivity: Human
Sensitivity: 0.188 mIU/ml
Range: 0.313 – 20 mIU/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TRACP-5b, no cross reactivity or interference between TRACP-5b  and analogues was detected.
– Recovery: Serum (85 – 105%), EDTA Plasma (90 – 105%), Heparin Plasma (87 – 104%).
– Linearity: Serum (87 – 100%), EDTA Plasma (82 – 100%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Tartrate-resistant acid phosphatase 5b (TRACP 5b) as a marker of bone resorption. Clin Lab. (2006) 52 (9-10): 499-509. Halleen J.M., et al.
2. The clinical utility of TRACP-5b to monitor anti-resorptive treatments of osteoporosis. Osteoporos Int. (2022) 33 (6): 1357-1363. Gossiel F., et al.
3. Exercise-induced hypercalcemia and vasopressin-mediated bone resorption. Osteoporos Int. (2021) 32 (12): 2533-2541. Senda M., et al.
4. Significance of serum TRACP in rheumatoid arthritis. J Bone Miner Res. (2008) 23 (8): 1287-95. Janckila A.J., et al.

ADDITIONAL INFORMATION

• Full Name: TRACP-5b ELISA Kit (TartRate-Resistant Acid Phosphatase 5b)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.188 mIU/ml

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