TRACP-5b ELISA Kit (TartRate-Resistant Acid Phosphatase 5b)

Full Name: TRACP-5b ELISA Kit (TartRate-Resistant Acid Phosphatase 5b)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.188 mIU/ml

INTRODUCTION

TRACP-5b, known as tartrate-resistant acid phosphatase 5b, is an enzyme used as a biomarker to assess bone resorption activity. Secreted by activated osteoclasts during bone remodeling, measuring serum TRACP-5b levels helps quantify skeletal dynamics in normal physiology and diseases like osteoporosis.

Bone continually remodels itself through osteoclast-mediated resorption of old tissue and osteoblast formation of new bone. Osteoclast activation secretes TRACP-5b to degrade and solubilize bone mineral to initiate skeletal framework teardown. Once released into circulation, serum TRACP-5b quantifies the current rate of bone matrix catabolism by skeletal osteoclasts.

Elevated serum TRACP-5b indicates heightened osteoclastic resorption outpacing bone formation. This imbalance favoring breakdown over regeneration reflects poorly controlled skeletal remodeling that weakens bone over time. In particular, postmenopausal women exhibit increased TRACP-5b levels in line with excessive resorption from estrogen-deficient bone loss. Monitoring serum TRACP-5b allows early detection of accelerated bone loss well before fragility fractures occur. The enzyme marker gauges patient response to osteoporosis therapies as well. Anti-resorptive treatments like bisphosphonates reduce TRACP-5b levels along with slowing skeletal deterioration, affirming osteoclast inhibition.

INTENDED USE

Human TRACP-5b ELISA kit can measure concentrations of tartRate-resistant acid phosphatase 5b (TRACP-5b, TRatPase, MGC117378) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti- TRACP-5b.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this TRACP-5b ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 mIU/ml.
Reactivity: Human
Sensitivity: 0.188 mIU/ml
Range: 0.313 – 20 mIU/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TRACP-5b, no cross reactivity or interference between TRACP-5b and analogues was detected.
– Recovery: Serum (85 – 105%), EDTA Plasma (90 – 105%), Heparin Plasma (87 – 104%).
– Linearity: Serum (87 – 100%), EDTA Plasma (82 – 100%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

Tartrate-resistant acid phosphatase 5b (TRACP 5b) as a marker of bone resorption. Clin Lab. (2006) 52 (9-10): 499-509. Halleen J.M., et al.
The clinical utility of TRACP-5b to monitor anti-resorptive treatments of osteoporosis. Osteoporos Int. (2022) 33 (6): 1357-1363. Gossiel F., et al.
Exercise-induced hypercalcemia and vasopressin-mediated bone resorption. Osteoporos Int. (2021) 32 (12): 2533-2541. Senda M., et al.
Significance of serum TRACP in rheumatoid arthritis. J Bone Miner Res. (2008) 23 (8): 1287-95. Janckila A.J., et al.

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