Transthyretin ELISA Kit (TTR)

INTENDED USE

Human transthyretin ELISA kit can measure concentrations of TTR (transthyretin, PALB, prealbumin, TBPA, amyloidosis type I) present is plasma, serum, biological fluids and tissue homogenate samples.

INTRODUCTION

Thyroxine and retinol, two significant blood components, are transported via transthyretin (TTR). Thyroxine controls growth, development and metabolism. Both the liver and the choroid plexus manufacture TTR. The brain has a structure that makes cerebrospinal fluid. Amyloidosis, a rare and dangerous disorder that develops when aberrant proteins build up in many organs and tissues and impede their function, is brought on by transthyretin. AL and ATTR are the two primary subtypes of TTR-amyloidosis. In the bone marrow, abnormal plasma cells that create abnormal light chains, which are pieces of antibodies, are the root cause of AL amyloidosis. These light chains create amyloid deposits, which can harm the kidneys, heart, nervous system and gastrointestinal tract. Multiple myeloma is a malignancy that has been linked to AL amyloidosis, which is not a hereditary condition.

The transthyretin gene mutations that result in ATTR amyloidosis make the protein unstable and more likely to develop amyloid deposits. You can either inherit or acquire ATTR amyloidosis. Acquired amyloidosis, also known as SSA or wild-type ATTR amyloidosis, affects mostly elderly males without a history of the condition in their families. The heart and nerves are the two organs that are most frequently impacted by amyloidosis. TTR-amyloidosis is a progressive and life-threatening condition that requires early diagnosis and treatment. Treatment options include medications that stabilize or reduce transthyretin production, organ transplantation, gene therapy and supportive care.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-TTR antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this TTR ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml.
Reactivity: Human
Sensitivity: 4.688 pg/ml
Range: 7.813 – 500 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TTR, no cross reactivity or interference between TTR and analogues was detected.
– Recovery: Serum (17 – 83%), EDTA Plasma (62 – 102%), Heparin Plasma (6 – 70%).
– Linearity: Serum (88 – 101%), EDTA Plasma (89 – 97%), Heparin Plasma (80 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Transthyretin cardiac amyloidosis: an update on diagnosis and treatment. ESC Heart Fail. (2019) 6 (6): 1128-1139. Yamamoto H. and Yokochi T.
2. Transthyretin-amyloid cardiomyopathy: An uncharted territory awaiting discovery. Eur J Intern Med. (2020) 82: 7-15. Porcari A., et al.
3. Cardiac_transthyretin amyloidosis. (2012) 98 (21): 1546-54. Dungu J.N., et al.
4. Transthyretin as a thyroid hormone carrier: function revisited. Clin Chem Lab Med. (2002) 40 (12): 1292-300. Palha J.A.

ADDITIONAL INFORMATION

• Full Name: Transthyretin ELISA Kit (TTR)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 4.688 pg/ml

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