Irisin ELISA Kit

Full Name: Irisin ELISA Kit
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Biological Fluids, Serum
Sensitivity: 0.938 pg/ml

INTRODUCTION

Irisin is made up of a fibronectin III (FNIII)-like region at the N-terminus and a flexible C-terminus. The FNIII-like domain creates a continuous inter subunit -sheet dimer, which has never been seen before in any FNIII protein. It controls bone remodelling and metabolism in both animal models and people. In reaction to exercise, it is secreted by the skeletal muscles and adipose tissue. It promotes energy expenditure by increasing browning of white adipose tissue and improves glucose balance by decreasing insulin resistance. Irisin is released from muscle and adipose tissue during exercise, it is unable to cross the blood-brain barrier because it is hydrophobic. It can circulate throughout the body in both an aqueous and a lipid phase since it is soluble in both water and lipids. It has less than 10% dissociation constant with biological membranes at physiological temperature.

Irisin is only active within 12 hours of release, so muscles must be continuously stimulated to keep working out their secreted irisin as they are constantly regenerating new muscle tissue. The amount that is released can be increased by high-intensity exercise. There are two types of irisin receptors; the alpha receptor, which responds to both the peptide and steroid hormones and the beta receptor, which responds to only steroids. The alpha receptor primarily mediates insulin’s effects on muscle, while the beta receptor has a role in metabolic rate. Irisin binds with both alpha and beta receptors and is secreted by skeletal muscle cells during periods of high-intensity exercise, aerobic activity, or mechanical stimulation. The binding also occurs in non-muscle tissue such as white adipose tissue, liver cells, and pancreatic β cells.

INTENDED USE

Human irisin ELISA kit can measure amounts of irisin present is serum, plasma, various biological fluids and tissue homogenate samples.

CONTENT

All reagents supplied with irisin ELISA kit need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti-irisin antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this irisin ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 1.562, 3.125, 6.25, 12.5, 25, 50, 100 ng/ml.
Reactivity: Human
Sensitivity: 0.938 ng/ml
Range: 1.563 – 100 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for irisin, no cross reactivity or interference between irisin and analogues was detected.
– Recovery: Serum (86 – 103%), EDTA Plasma (91 – 100%), Heparin Plasma (87 – 99%).
– Linearity: Serum (93 – 103%), EDTA Plasma (87 – 105%), Heparin Plasma (83 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

Irisin Mediates Effects on Bone and Fat via αV Integrin Receptors. (2018) 175 (7): 1756-1768.e17. Kim H., et al.
Role of Irisin in Myocardial Infarction, Heart Failure, and Cardiac Hypertrophy. (2021) 10 (8): 2103. Ho M.Y. and Wang C.Y.
Involvement of GPX4 in irisin’s protection against ischemia reperfusion-induced acute kidney injury. J Cell Physiol. (2021) 236 (2): 931-945. Zhang J., et al.
Irisin: A bridge between exercise and neurological diseases. Zhang X, Lin S. (2022) 8 (12): e12352. Zhang Y., et al.

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