Thromboxane B2 ELISA Kit (TXB2)

Full Name: Thromboxane B2 ELISA Kit (TXB2)
Reactivity: Universal
Sample Type: Plasma, Tissue Homogenates, Biological Fluids, Serum
Sensitivity: 0.938 pg/ml

INTRODUCTION

Thromboxane B2 is a thromboxane A metabolite. It is nearly entirely eliminated in the urine. Aspirin, which suppresses the COX-1 enzyme and thus lowers thromboxane A2 levels, targets its synthesis. It is a steady thromboxane A2 degradation product that is excreted by the kidneys as 11-dehydro TXB-2 (d-TXB 2). It causes arterial constriction as well as platelet clumping and aggregation. A long-term inflammatory condition of the artery wall called atherosclerosis frequently results in increased cholesterol being accumulated in the arteries. TXB2 is a pro-inflammatory substance produced by macrophages, is reportedly essential for the formation of atherosclerotic plaque. It also encourages leukocyte transit across it and strengthens their cling to endothelial cells, both of which contribute to atherosclerosis.

Thromboxane B2 does not play a role in platelet stimulation and aggregation in the presence of a wound, but its precursor, thromboxane A2, does. TXB-2 levels in the blood correspond with platelet COX-1 activation and TXB-2 synthesis. Platelets respond to increased levels of TXB2 by decreasing synthesis of TXA2, which in turn leads to decreased aggregation, or the ability of platelets to stick together and help create a clot. This is important because when platelets are activated they are more likely to aggregate, which can lead to clots in arteries, causing a heart attack and stroke. TXB A1 deficiency at the site of a wound may lead to undesirable effects such as retention or accumulation of fluid within the wound space so that it cannot be drained and treated effectively.

INTENDED USE

Thromboxane B2 ELISA kit is ideal for analysing levels of TXB2 (thromboxane B2) present is various biological fluids, serum, tissue homogenate and plasma samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- TXB2 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this thromboxane B2 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 1.562, 3.125, 6.25, 12.5, 25, 50, 100 pg/ml.
Reactivity: Universal
Sensitivity: 0.938 pg/ml
Range: 1.563 – 100 pg/ml
Principle: Competitive
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TXB2, no cross reactivity or interference between TXB2 and analogues was detected.
– Recovery: Serum (86 – 103%), EDTA Plasma (86 – 104%), Heparin Plasma (87 – 103%).
– Linearity: Serum (85 – 103%), EDTA Plasma (80 – 100%), Heparin Plasma (80 – 91%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. The Role of Thromboxane in the Course and Treatment of Ischemic Stroke: Review. Int J Mol Sci. (2021) 22 (21): 11644. Szczuko M., et al.
2. Urinary 11-dehydro-thromboxane B2 levels are associated with vascular inflammation and prognosis in atherosclerotic cardiovascular disease. Prostaglandins Other Lipid Mediat. (2018) 134: 24-31. Wang N., et al.
3. A monoclonal anti-thromboxane B2 antibody. FEBS Lett. (1988) 232 (1): 46-50. Reinke M. and Brune K.
4. Enzyme-immunoassay of thromboxane B2 at the picogram level. (1985) 29 (6): 1039-48. Sawada M., et al.

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• Hepcidin ELISA Kit
• Transthyretin ELISA Kit
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