Total Estriol ELISA Kit

INTENDED USE

Human total estriol ELISA kit is a reliable quantitative procedure for measuring total estriol in human serum or plasma samples. This assay has a minimum sensitivity detection limit of 0.05 ng/ml.

BACKGROUND

Estriol (E3 or oestriol) is a female sex steroid hormone and is member of the naturally occurring estrogens which also include estrone and estradiol. It is a major estrogen that is produced by the human fetus and estriol is also exclusively produced during pregnancy. The production by Fetal-placenta results in a progressive elevation in maternal circulating levels which reaches a late-gestational peak that can be over many orders of magnitude greater than non-pregnant concentrations. DHEA is also produced by the adrenal cortex of the fetus and this can be converted to estriol by the placenta.

In non-pregnant individuals estriol is known to be derived almost exclusively from 17 β-Estradiol and it exists in the biological matrix has either a conjugated form (approx. 92%) or as a free hormone (approx. 8%). The process of conjugation helps steroid solubility and allows rapid elimination through the kidney, conjugation occurs in the liver with glucuronic or sulphuric acid.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Coated Microplate (1 breakable microplate): Contains anti-estriol antibody adsorbed on microplate.
• Standards 0-4: Concentrations 0, 2, 20, 80, 200 ng/ml.
• Controls 1 And Control 2: Containing human estriol.
• Enzyme Conjugate: Consisting of antibody conjugated with horseradish peroxidase (HRP).
• TMB Substrate Solution.
• Stop Solution: Sulphuric acid.
• Wash Solution (10x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of estriol (total) using this total estriol ELISA kit was approximately 0.05 ng/ml. The dynamic range for this assay is 2.0 – 200.0 ng/ml.

ASSAY CHARACTERISTICS

– Intra-Assay Variation: Assay variability is 9.9%.
– Inter-Assay Variation: Assay variability is 10.3%.
– Sensitivity: 1.05 ng/mL
– Specificity (Cross Reactivity): Estriol (100 %), 16 epi-estriol (10.5 %), 15 αOH-estriol (7.0 %),  Estriol 3 Sulphate (2.0 %),  Estradiol (0.1 %), 17 epi-estriol, Estriol 3α Glucoronate, Estriol 16α Glucoronate (< 1 x 10-2 %), Estrone (< 1 x 10-4 %).
– Interfering Substances: None

REFERENCES

1. Clinical use of plasma total estriol-measurements in late pregnancy. J Reprod Med. (1979) 23 (4): 179-84. Hagerman D.D.
2. Cardioprotection by estrogens: mechanisms of action–the lipids. Int J Fertil Menopausal Stud. (1994) 39 Suppl 1: 43-9. Review. Samsioe G.
3. Total urine oestriol by a modification of a commercial plasma oestriol kit. Ann Clin Biochem. (1978) 15 (6): 338-9. Seddon R.M. and Attwood E.C.
4. Comparison of total urinary oestrogens and total serum oestriol in pregnancy. Clin Chim Acta. (1978) 86 (3): 357-67. Wolfrum R.

ADDITIONAL INFORMATION

• Full Name: Total Estriol ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity: 0.05 ng/ml

OTHER RELATED ELISA KITS

• Estradiol E2 ELISA Kit
• Estrone ELISA Kit
• 17 Beta Estradiol ELISA Kit