Estradiol E2 ELISA Kit

INTENDED USE

Human estradiol E2 ELISA kit is a reliable quantitative procedure for measuring estradiol E2 levels in human serum samples. This assay has a minimum sensitivity detection of 10.0 pg/ml.

BACKGROUND

Estradiol E2 is a steroid hormone that has a molecular weight of 272.4 Da, it contains a phenolic A ring. It is a natural estrogen which can be produced predominately by; the placenta, ovary, male testes and adrenal cortex. Over 98% of it is secreted into the bloodstream is found to circulate attached to the sex hormone binding globulin (SHBG) and to a less extent to other proteins like albumin. This means that only a very small faction circulates in the conjugated form or as a free hormone form.

There are estradiol-receptor complexes which are responsible for stimulating the appropriate response at the nuclear level within the specific the target sites. There are many target sites which can include; follicles, breast, uterus, urethra, vagina, pituitary and hypothalamus. Measuring serum levels can be essential in helping to evaluate a number of different menstrual dysfunctions for example precocious, menopause, delayed puberty in girls, primary and secondary amenorrhea. Elevated levels have also be identified in patients with gynaecomastia, testicular tumors and feminizing syndromes.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterwells 12 x 8 (break apart) strips: Wells coated with anti estradiol antibody.
• Standards: Concentration 0 – 1,000.0 pg/ml.
• Control Low And Control High.
• Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase.
• Substrate Solution: Tetramethylbenzidine (TMB).
• Stop Solution: Contains 0.5 M H2SO4, Avoid contact with the stop solution. It may cause skin irritations and burns.
• Wash Solution (40x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of estradiol E2 using current estradiol-E2 ELISA kit was approximately 10.0 pg/ml. The dynamic range for this assay is 10.0 – 1,000.0 pg/ml.

REFERENCES

1. Correlation between estradiol and progesterone in cycles with luteal phase deficiency. Fertil. Steril. (1982) 37: 348-354. Goldstein D., et al.
2. The serum transport of steroid hormones, Rec. Prog. Horm. Res. (1982) 38: 457-510. Siiteri P.K., et al.
3. Steroid biosynthesis by isolated human ovarian follicular cells in vitro, J. Clin. Endocrinol. Metab. (1980) 51: 1407-1411. Tsang B.K., Armstrong D.T. and Whitfield J.F.
4. Oestradiol assays: applications and guidelines for the provision of a clinical biochemistry service, Ann. Clin. Biochem. (1988) 25:466-483. Ratcliffe W.A.,et al.

ADDITIONAL INFORMATION

• Full Name: Estradiol E2 ELISA Kit
• Reactivity: Human
• Sample Type: Serum
• Sensitivity: 10.0 pg/ml

OTHER RELATED ELISA KITS

• Estrone ELISA Kit
• 17 Beta Estradiol ELISA Kit
• Free Beta hCG ELISA Kit