TNF Alpha ELISA Kit (Rat)


Rat TNF alpha ELISA kit is designed for analysing quantitative amounts of rat tumor necrosis factor alpha (TNF-a, TNF-α, TNF-alpha) using cell culture supernatant. This assay has a minimum sensitivity detection limit of 10.0 pg/ml.


Tumor necrosis factor-alpha (TNF-alpha, TNF-a, TNF alpha) is a potent lymphoid factor which is approx. 17.5kDa in size and is composed of 157 amino acids. TNF-alpha play a major role as a pro-inflammatory factor, it can also exert cytotoxic effects on many tumor cells and has been found to be present in the synovial fluids of rheumatoid arthritis patients. TNF-a biosynthesis is tightly regulated where it is produced in small amounts in quiescent cells, but secreted in large quantities in cells that are activated.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Plate: Anti-rat TNF-α (C) rabbit IgG.
  • Labelled Antibody (30x Conc.).
  • Standards (0-8): Recombinant rat TNF-alpha, concentrations 0, 56.25, 112.5, 225, 450, 900, 1,800, 3,600 pg/ml.
  • EIA Buffer.
  • Solution For Labelled Antibody.
  • Chromogen Solution (TMB).
  •  Stop Solution.
  • Wash Buffer (Concentrated).


The minimum sensitivity detection limit of tumor necrosis factor alpha (TNF-α, TNF-a) using current rat TNF-alpha ELISA kit is 10.0 pg/ml. The dynamic range for this assay is 56.0 – 3,600.0 pg/ml. It has the ability to detect both recombinant and native rat TNF-alpha.


– Rat Tumor Necrosis Factor Alpha (TNF alpha): ELISA
– Titer Assay: 87 – 102.4%
– Added Recovery Assay: 82.3 – 86.4%
– Intra – Assay: 4.3 – 6.5%
– Inter – Assay: 6.3 – 10.2%
– Specificity (Cross Reactivity):  Rat TNF-α (100 %), Rat IL-6 (≦ 0.1 %), Rat MCP-1 (≦ 0.1 %), Rat Osteopontin (≦ 0.1 %), Rat CINC-1 (≦ 0.1 %).
– Sensitivity: 10.55 pg/ml


  1. The effects of anti-TNF-α antibody on hyper-prolactinemia-related suppression of hCG-induced testosterone release in male rats. J Sex Med. (2012) 9 (4): 1005-14. Huang W.J., et al.
  2. Roles of TNF-α, GSK-3β and RANKL in the occurrence and development of diabetic osteoporosis. Int J Clin Exp Pathol. (2015) 8 (10): 11995-2004. Qi J., et al.
  3. Rat tumour necrosis factor-alpha: expression in recombinant Pichia pastoris, purification, characterization and development of a novel ELISA. Eur Cytokine Netw. (1999) 10 (3): 383-92. Rees G.S., et al.
  4. The role of TNFα in the periaqueductal gray during naloxone-precipitated morphine withdrawal in rats. Neuro-psychopharmacology. (2011) 36 (3): 664-76. Hao S., et al.


  • Full Name: Tumor Necrosis Factor Alpha ELISA Kit
  • Gene Name: TNF Alpha ELISA Kit
  • Reactivity: Rat
  • Sample Type: Cell Supernatant
  • Sensitivity: 10.0 pg/ml



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford